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首页> 外文期刊>Journal of Clinical Microbiology >Detection of a Novel Strain of Porcine Circovirus in Pigs with Postweaning Multisystemic Wasting Syndrome
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Detection of a Novel Strain of Porcine Circovirus in Pigs with Postweaning Multisystemic Wasting Syndrome

机译：断奶后多系统衰竭综合征猪新的猪圆环病毒株的检测

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Swine infectious agents, especially viruses, are potential public health risks associated with the use of pig organs for xenotransplantation in humans. Therefore, there is a need for better characterization of swine viruses and for the development of diagnostic tests for their detection. We report here isolation of a novel strain of porcine circovirus (PCV) from pigs with postweaning multisystemic wasting syndrome (PMWS). Affected pigs exhibited severe interstitial pneumonia and lymphoid depletion. The complete nucleotide sequence (1,768 nucleotides) of the genome of the PCV isolate was determined and compared with the sequence of the PCV strain isolated from PK-15 cells. Sequence comparison revealed significant differences between the two PCV strains, with an overall DNA homology of 76%. Two major open reading frames (ORFs) were identified. ORF1 was more conserved between the two strains, with 83% nucleotide homology and 86% amino acid homology. ORF2 was more variable, with nucleotide homology of 67% and amino acid homology of 65%. PCR and in situ hybridization demonstrated abundant viral DNA in various organs of pigs with PMWS. In situ hybridization demonstrated that this strain of PCV targets multiple organs and infects macrophages, lymphocytes, endothelial cells, and epithelial cells.
机译：猪的传染源，尤其是病毒，是与使用猪器官进行人类异种移植相关的潜在公共卫生风险。因此，需要更好地表征猪病毒并开发用于检测其的诊断测试。我们在此报告从断奶后多系统消耗综合症（PMWS）的猪中分离出一种新型猪圆环病毒（PCV）株。患病猪表现出严重的间质性肺炎和淋巴样耗竭。确定了PCV分离株基因组的完整核苷酸序列（1,768个核苷酸），并将其与从PK-15细胞分离出的PCV菌株的序列进行了比较。序列比较揭示了两个PCV毒株之间的显着差异，总体DNA同源性为76％。确定了两个主要的开放阅读框（ORF）。 ORF1在两个菌株之间更保守，核苷酸同源性为83％，氨基酸同源性为86％。 ORF2的可变性更大，核苷酸同源性为67％，氨基酸同源性为65％。 PCR和原位杂交表明，PMWS能在猪的各个器官中提供丰富的病毒DNA。原位杂交表明，该PCV菌株靶向多个器官并感染巨噬细胞，淋巴细胞，内皮细胞和上皮细胞。

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