Identification of Pneumocystis cariniif. sp. hominis Gene Sequences in Filtered Air in Hospital Environments

Mats Olsson; Christer Lidman; Sophie Latouche; Anders Bj?rkman; Patricia Roux; Ewert Linder; Mats Wahlgren

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Identification of Pneumocystis cariniif. sp. hominis Gene Sequences in Filtered Air in Hospital Environments

机译：鉴定卡氏肺孢子虫。 sp。医院环境中过滤空气中的人型基因序列

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To evaluate the risk of a nosocomial spread of Pneumocystis carinii f. sp. hominis (P. carinii hominis), air filter samples from rooms of P. cariniipneumonia (PCP) patients, adjacent corridors, and other hospital environments have been investigated for the presence of P. carinii hominis. Amplified DNA from air filters and sputum or bronchoalveolar lavage samples from the PCP patients have been genotyped with the P. carinii hominis genes of the mitochondrial large-subunit (mtLSU) rRNA and the internal transcribed spacers (ITS1 and ITS2) of the rRNA. Genotypes of the two loci were identified by direct sequencing, and for site 85 of the mtLSU locus, three allele-specific PCR assays were used. P. carinii hominis DNA was identified in the air of five of seven PCP patient rooms and in the air of two of four air filtrations from the ward corridors. The P. carinii hominis genotypes were the same in four of the five room air samples as those in the corresponding patients, suggesting a risk of person-to-person transmission ofP. carinii hominis from PCP patients. Three of 16 air samples collected in infectious disease wards without the presence of PCP patients and one sample from a cardiology unit in a separate hospital building were also positive, which further strengthens the possibility of acquisition of P. carinii hominis from the environment.

机译：评估卡氏肺孢子虫 f在医院内传播的风险。 sp。 hominis （ carinii hominis ），来自 P房间的空气过滤器样品。对卡林氏肺炎（PCP）患者，邻近走廊和其他医院环境进行了调查，确定是否存在 P。 Carinii Hominis 。来自肺过滤器和来自PCP患者的痰液或支气管肺泡灌洗液样品的扩增DNA已通过 P基因分型。线粒体大亚基（mtLSU）rRNA的carinii hominis 基因和rRNA的内部转录间隔区（ITS1和ITS2）。通过直接测序鉴定了两个基因座的基因型，对于mtLSU基因座的位点85，使用了三个等位基因特异性PCR分析法。 P。在七个PCP病房中的五个中，以及在病房走廊中四个滤清器中的两个中，鉴定出人卡里纳氏菌DNA。 P。在五个室内空气样本中，有四个样本的卡林氏人型基因型与相应患者的相同，这表明人传人 P的风险。来自PCP患者的carinii hominis 。在没有PCP患者的情况下在传染病病房中收集的16个空气样本中的3个以及在单独医院大楼中的心脏病科收集的一个样本也呈阳性，这进一步增加了获得 P的可能性。来自环境的carinii hominis 。 展开▼

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《Journal of Clinical Microbiology》 |1998年第6期|共4页

作者
Mats Olsson; Christer Lidman; Sophie Latouche; Anders Bj?rkman; Patricia Roux; Ewert Linder; Mats Wahlgren;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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专利

1. DNA sequences identical to Pneumocystis carinii f. sp. carinii and Pneumocystis carinii f. sp. hominis in samples of air spora. [J] . A E Wakefield Journal of Clinical Microbiology . 1996,第7期

机译：与卡氏肺孢子虫相同的DNA序列。 sp。卡林氏和卡氏肺孢子虫f。 sp。空气孢子样本中的人类。

2. Genetic Diversity of Pneumocystis carinii f. sp. hominis Based on Variations in Nucleotide Sequences of Internal Transcribed Spacers of rRNA Genes [J] . Laila F. Nimri, Iaci N. S. Moura, Laurence Huang, Journal of Clinical Microbiology . 2002,第4期

机译：卡氏肺孢子虫的遗传多样性sp。基于rRNA基因内部转录间隔区核苷酸序列变异的人

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机译：卡氏肺孢子虫基因分型f。 sp。基于日本rRNA基因内部转录间隔区中核苷酸序列的变异，在日本分离了人类原始人。

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6. Identification of Pneumocystis carinii f. sp. hominis Gene Sequences in Filtered Air in Hospital Environments [O] . Mats Olsson, Christer Lidman, Sophie Latouche, 1998

机译：卡氏肺孢子虫的鉴定f。 sp。医院环境中过滤空气中的人型基因序列

7. Identification of Pneumocystis carinii f. sp. hominis Gene Sequences in Filtered Air in Hospital Environments [O] . Mats Olsson, Christer Lidman, Sophie Latouche, 1998

机译：胸膜炎术鉴定Carinii F. sp。在医院环境中过滤空气中的Hominis基因序列

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Identification of Pneumocystis cariniif. sp. hominis Gene Sequences in Filtered Air in Hospital Environments

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