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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Epidemiology of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Isolates in a District Hospital in Taiwan
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Molecular Epidemiology of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Isolates in a District Hospital in Taiwan

机译：台湾地区医院产广谱β-内酰胺酶的肺炎克雷伯菌的分子流行病学研究

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Thirty-one of 104 clinical isolates of Klebsiella pneumoniae collected over a period of 8 months were found to be putative extended-spectrum β-lactamase (ESBL) producers. Isoelectric focusing and an iodine overlay agar method were used for preliminary identification of the ESBLs. They were further identified by DNA sequencing. Seventy-one percent of the isolates were found to produce SHV-5. The variation in the ESBL patterns of these isolates was slight, with only five patterns being identified. The strains were typed by pulsed-field gel electrophoresis (PFGE), and 16 different genotypes were identified. When the PFGE patterns were analyzed by the algorithmic clustering method called the unweighted-pair group method using arithmetic averages, five clusters were found. However, significant genetic variations were found among 11 isolates and between each cluster. A plasmid of 36 kb was found in all clinical isolates and in the transconjugants. Our results indicate that the increase in the number of ESBL-producing K. pneumoniae isolates in this hospital is due mainly to the dissemination of a resistance plasmid rather than to the clonal spread of a few epidemic strains.
机译：在8个月的时间内收集到的104株肺炎克雷伯菌临床分离株中有31株是假定的广谱β-内酰胺酶（ESBL）生产者。等电聚焦和碘覆盖琼脂法用于ESBLs的初步鉴定。通过DNA测序进一步鉴定它们。发现有71％的分离株产生SHV-5。这些分离株的ESBL图谱变化很小，仅鉴定出五个图谱。通过脉冲场凝胶电泳（PFGE）对菌株进行分型，并鉴定出16种不同的基因型。当使用算术平均值通过称为非加权对组方法的算法聚类方法分析PFGE模式时，发现了五个聚类。然而，在11个分离株之间以及每个簇之间发现了显着的遗传变异。在所有临床分离株和转导结合物中都发现了一个36 kb的质粒。我们的结果表明，产生ESBL的 K数量增加。在该医院中分离到的肺炎主要是由于抗药性质粒的传播，而不是一些流行毒株的克隆传播。

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