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首页> 外文期刊>Journal of Clinical Microbiology >Inducible β-Lactam Resistance in Aeromonas hydrophila: Therapeutic Challenge for Antimicrobial Therapy
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Inducible β-Lactam Resistance in Aeromonas hydrophila: Therapeutic Challenge for Antimicrobial Therapy

机译：嗜水气单胞菌的诱导型β-内酰胺抗性：抗菌治疗的治疗挑战

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Despite the abundant amount of knowledge about inducible chromosomally mediated β-lactamases among Aeromonasspecies, extended-spectrum β-lactam-resistant A. hydrophila strains selected in clinical practice were rarely reported. In the present study, two strains of A. hydrophila, A136 and A139, with markedly different susceptibilities to extended-spectrum cephalosporins were isolated from blood and the tip segment of an arterial catheter of a burn patient. Another strain (A136m) was selected in vitro by culturing A136 in a subinhibitory concentration of cefotaxime, the β-lactam agent administered for the treatment of Aeromonas bacteremia in this patient. Typing studies by arbitrarily primed PCR and pulsed-field gel electrophoresis indicated a clonal relationship among strains A136, A136m, and A139. These strains were identified to be of DNA hybridization group 1. Wild-type strain A136 was resistant only to ampicillin and cephamycins, but A136m and A139 were highly resistant to the expanded- and broad-spectrum cephalosporins. The presence of increased β-lactamase activity in A139 suggests that A139 is a derepressed mutant which overexpresses β-lactamases. These results call attention to the use of β-lactam agents for the treatment of invasive Aeromonas infections.
机译：尽管对气单胞菌物种中可诱导的染色体介导的β-内酰胺酶有丰富的知识，但广谱的β-内酰胺抗性 A。在临床实践中选择的嗜水菌菌株鲜有报道。在本研究中，两个菌株是 A。从烧伤患者的血液和动脉导管的尖端中分离出对大范围头孢菌素敏感性明显不同的亲水性A136和A139。通过在亚抑制浓度的头孢噻肟（一种用于治疗该患者的气单胞菌菌血症的β-内酰胺剂）浓度下培养A136，体外选择了另一株菌株（A136m）。通过任意引物PCR和脉冲场凝胶电泳的打字研究表明，菌株A136，A136m和A139之间存在克隆关系。这些菌株被鉴定为属于DNA杂交组1。野生型菌株A136仅对氨苄青霉素和头孢霉素具有抗性，而A136m和A139对扩展的广谱头孢菌素具有高度抗性。在A139中存在β-内酰胺酶活性增加表明A139是过表达β-内酰胺酶的去抑制的突变体。这些结果引起人们对使用β-内酰胺类药物治疗侵袭性气单胞菌感染的关注。

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