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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Decreased Fluoroquinolone Susceptibility in Salmonellas and Validation of Nalidixic Acid Screening Test
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Detection of Decreased Fluoroquinolone Susceptibility in Salmonellas and Validation of Nalidixic Acid Screening Test

机译：沙门氏菌中氟喹诺酮敏感性降低的检测和萘啶酸筛选试验的验证

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We evaluated 1,010 Salmonella isolates classified as fluoroquinolone susceptible according to the National Committee for Clinical Laboratory Standards guidelines for susceptibility to nalidixic acid and three fluoroquinolones. These isolates were divided into two distinct subpopulations, with the great majority (n = 960) being fully ciprofloxacin susceptible and a minority (n = 50) exhibiting reduced ciprofloxacin susceptibility (MICs ranging between 0.125 and 0.5 μg/ml). The less ciprofloxacin-susceptible isolates were uniformly resistant to nalidixic acid, while only 12 (1.3%) of the fully susceptible isolates were nalidixic acid resistant. A similar association was observed between resistance to nalidixic acid and decreased susceptibility to ofloxacin or norfloxacin. A mutation of the gyrA gene could be demonstrated in all isolates for which the ciprofloxacin MICs were ≥0.125 μg/ml and in 94% of the nalidixic acid-resistant isolates but in none of the nalidixic acid-susceptible isolates analyzed. Identification of nalidixic acid resistance by the disk diffusion method provided a sensitivity of 100% and a specificity of 87.3% as tools to screen for isolates for which the MICs of ciprofloxacin were ≥0.125 μg/ml. We regard it as important that microbiology laboratories endeavor to recognize these less susceptibleSalmonella strains, in order to reveal their clinical importance and to survey their epidemic spread.
机译：我们根据国家临床实验室标准委员会关于萘啶酸和三种氟喹诺酮类药物敏感性的指南，评估了1,010种沙门氏菌沙门氏菌菌株，这些菌株被归类为氟喹诺酮类易感药物。这些分离物分为两个不同的亚群，绝大多数（ n = 960）完全对环丙沙星敏感，而少​​数（ n = 50）表现出环丙沙星敏感性降低（MICs）范围介于0.125和0.5μg/ ml之间）。环丙沙星敏感性较低的分离株对萘啶酸具有统一的抗药性，而完全敏感的分离株中只有12种（1.3％）对萘啶酸具有抗药性。在对萘啶酸的抗性和对氧氟沙星或诺氟沙星的敏感性降低之间观察到相似的关联。可以在所有环丙沙星MICs≥0.125μg/ ml的分离株和94％耐萘啶酸的分离株中证实 gyrA 基因的突变，但没有对萘啶酸敏感的分离株分离物进行了分析。通过圆盘扩散法鉴定耐萘啶酸的能力，提供了100％的灵敏度和87.3％的特异性，可用于筛选环丙沙星的MIC≥0.125μg/ ml的分离株。我们认为微生物实验室必须努力识别这些较不易感染的沙门氏菌菌株，以揭示其临床重要性并调查其流行程度，这一点很重要。

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