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首页> 外文期刊>Journal of Clinical Microbiology >Comparative Evaluation of Nine Different Enzyme-Linked Immunosorbent Assays for Determination of Antibodies againstTreponema pallidum in Patients with Primary Syphilis
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Comparative Evaluation of Nine Different Enzyme-Linked Immunosorbent Assays for Determination of Antibodies againstTreponema pallidum in Patients with Primary Syphilis

机译：九种不同酶联免疫吸附法测定原发性梅毒患者抗梅毒螺旋体抗体的比较评价

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摘要

Nine different enzyme-linked immunosorbent assays (ELISAs) with a sonicate or recombinant proteins of Treponema pallidum as antigen have been evaluated comparatively by testing 52 highly selected sera from patients with primary syphilis, all negative in the microhemagglutination test for T. pallidum (MHA-TP). Eight tests exhibited greater sensitivity (48.5 to 76.9%) than the commonly used Venereal Disease Research Laboratory test (44.2%). Higher sensitivity could be related to (i) the volume and dilution of the serum, (ii) the design of the assay (capture and competitive tests showed higher sensitivity than sandwich-based assays), and (iii) the ability to detected specific immunoglobulin M antibodies. The specificity of the ICE Syphilis and the Enzygnost Syphilis tests was 99.5 and 99.8%, respectively, as determined by routine testing of 2,053 unselected sera in comparison with the MHA-TP test. ELISAs tested offered high sensitivity in patients with primary syphilis; however, recommendations to use these tests as screening assays do need further data on specificity and reactivity in late stages of the disease.
机译：通过测试52种从原发性梅毒患者中精选出的血清进行了比较，评估了九种不同的酶联免疫吸附测定（ELISA），其中超声或梅毒螺旋体重组蛋白为抗原，在微血凝试验中均为阴性对于 T。苍白球（MHA-TP）。八项测试显示出比常用的性病研究实验室测试（44.2％）更高的灵敏度（48.5至76.9％）。更高的灵敏度可能与（i）血清的体积和稀释度，（ii）分析的设计（捕获和竞争性测试显示出比基于三明治的分析更高的灵敏度）以及（iii）检测特异性免疫球蛋白的能力有关。 M抗体。 ICE梅毒测试和Enzygnost梅毒测试的特异性分别为2053次未选择的血清与MHA-TP测试的常规测试，分别为99.5和99.8％。测试的ELISA对原发性梅毒患者具有很高的敏感性;然而，使用这些检测作为筛查分析的建议确实需要有关疾病晚期特异性和反应性的进一步数据。

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