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首页> 外文期刊>Journal of Clinical Microbiology >Genetic Structure of Population of Bacillus cereus and B. thuringiensis Isolates Associated with Periodontitis and Other Human Infections
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Genetic Structure of Population of Bacillus cereus and B. thuringiensis Isolates Associated with Periodontitis and Other Human Infections

机译：与牙周炎和其他人类感染有关的蜡状芽孢杆菌和苏云金芽孢杆菌菌株的种群遗传结构

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The genetic diversity and relationships among 35 Bacillus cereus and Bacillus thuringiensis isolates recovered from marginal and apical periodontitis in humans and from various other human infections were investigated using multilocus enzyme electrophoresis. The strains were isolated in Norway, except for three strains isolated from periodontitis patients in Brazil. The genetic diversity of these strains was compared to that of 30 isolates from dairies in Norway and Finland. Allelic variation in 13 structural gene loci encoding metabolic enzymes was analyzed. Twelve of the 13 loci were polymorphic, and 48 unique electrophoretic types (ETs) were identified, representing multilocus genotypes. The mean genetic diversity among the 48 genotypes was 0.508. The genetic diversity of each source group of isolates varied from 0.241 (periodontal infection) to 0.534 (dairy). Cluster analysis revealed two major groups separated at a genetic distance of greater than 0.6. One cluster, ETs 1 to 13, included solely isolates from dairies, while the other cluster, ETs 14 to 49, included all of the human isolates as well as isolates from dairies in Norway and Finland. The isolates were serotyped using antiflagellar antiserum. A total of 14 distinct serotypes were observed. However, little association between serotyping and genotyping was seen. Most of the strains were also analyzed with pulsed-field gel electrophoresis, showing the presence of extrachromosomal DNA in the size range of 15 to 600 kb. Our results indicate a high degree of heterogeneity among dairy strains. In contrast, strains isolated from humans had their genotypes in one cluster. Most strains from patients with periodontitis belonged to a single lineage, suggesting that specific clones of B. cereus and B. thuringiensis are associated with oral infections.
机译：利用多位点酶电泳技术研究了从人的边缘和根尖牙周炎以及其他各种人类感染中回收的35株蜡状芽孢杆菌和苏云金芽孢杆菌的遗传多样性及其相互关系。除从巴西的牙周炎患者中分离出的三株菌株外，在挪威分离出了这些菌株。将这些菌株的遗传多样性与挪威和芬兰的30个奶牛场分离株的遗传多样性进行了比较。分析了13个编码代谢酶的结构基因位点的等位变异。 13个基因座中有12个是多态性的，并且鉴定出48个独特的电泳类型（ET），代表多位点基因型。 48个基因型之间的平均遗传多样性为0.508。每个来源的分离株的遗传多样性从0.241（牙周感染）到0.534（乳制品）不等。聚类分析显示，两个主要群体的遗传距离大于0.6。一个集群，ET 1至13，仅包括奶牛场的分离株，而另一个集群，ET 14至49，包括所有人类分离株，以及挪威和芬兰的奶牛场。使用抗鞭毛抗血清对分离株进行血清分型。总共观察到14种不同的血清型。然而，血清型和基因型之间几乎没有关联。大多数菌株还用脉冲场凝胶电泳进行了分析，显示存在染色体外DNA，大小在15至600 kb之间。我们的结果表明，乳品菌株之间存在高度异质性。相反，从人类分离出的菌株的基因型集中在一个簇中。来自牙周炎患者的大多数菌株属于单个谱系，表明 B的特定克隆。蜡状菌和 B。苏云金与口腔感染有关。

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