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首页> 外文期刊>Journal of Clinical Microbiology >Pandemic Spread of an O3:K6 Clone of Vibrio parahaemolyticus and Emergence of Related Strains Evidenced by Arbitrarily Primed PCR and toxRS Sequence Analyses
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Pandemic Spread of an O3:K6 Clone of Vibrio parahaemolyticus and Emergence of Related Strains Evidenced by Arbitrarily Primed PCR and toxRS Sequence Analyses

机译：通过任意引物PCR和toxRS序列分析证明副溶血性弧菌O3：K6克隆的大流行和相关菌株的出现

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摘要

Vibrio parahaemolyticus O3:K6 strains responsible for the increase in the number of cases of diarrhea in Calcutta, India, beginning in February 1996 and those isolated from Southeast Asian travelers beginning in 1995 were shown to belong to a unique clone characterized by possession of the tdh gene but not thetrh gene and by unique arbitrarily primed PCR (AP-PCR) profiles (J. Okuda, M. Ishibashi, E. Hayakawa, T. Nishino, Y. Takeda, A. K. Mukhopadhyay, S. Garg, S. K. Bhattacharya, G. B. Nair, and M. Nishibuchi, J. Clin. Microbiol. 35:3150–3155, 1997). Evidence supporting a hypothesis that this clone emerged only recently and is spreading to many countries was obtained in this study. Of 227 strains isolated in a hospital in Bangladesh between 1977 and 1998, only 22 strains isolated between 1996 and 1998 belonged to the new O3:K6 clone (defined by the serovar, the tdh andtrh typing, and AP-PCR profiles). The O3:K6 strains isolated from clinical sources in Taiwan, Laos, Japan, Thailand, Korea, and the United States between 1997 and 1998 were also shown to belong to the new O3:K6 clone. The clonality of the new O3:K6 strains was also confirmed by analysis of the toxRS sequence, which has been shown to be useful for phylogenetic analysis of the members of the genus Vibrio. The toxRS sequences of the representative strains of the new O3:K6 clone differed from those of the O3:K6 strains isolated before 1995 at least at 7 base positions within a 1,346-bp region. A new PCR method targeted to 2 of the base positions unique to the new O3:K6 clone was developed. This PCR method could clearly differentiate all 172 strains belonging to the new O3:K6 clone from other O3:K6 strains isolated earlier. One hundred sixty-six strains belonging to 28 serovars other than O3:K6 were also examined by the new PCR method. The tdh-positive andtrh-lacking strains that belonged to the O4:K68 and O1:K untypeable serovars and were isolated in three countries and from international travelers beginning in 1997 gave positive results. The AP-PCR profiles of these strains were nearly identical to those of the new O3:K6 clone, and their toxRS sequences were 100% identical to that of the new O3:K6 clone. The results suggest that these strains may have diverged from the new O3:K6 clone by alteration of the O:K antigens. In conclusion, this study presents strong evidence for the first pandemicity in the history of V. parahaemolyticus and reports a novel toxRS-targeted PCR method that will be useful in epidemiological investigation of the cases associated with the current pandemic spread.
机译：从1996年2月开始，导致印度加尔各答腹泻病例增加的副溶血弧菌 O3：K6菌株和从1995年开始从东南亚旅行者中分离出来的菌株被证明是独特的克隆的特征是拥有 tdh 基因而不是 trh 基因，并且具有独特的任意引物PCR（AP-PCR）谱（J. Okuda，M。Ishibashi，E。 Hayakawa，T. Nishino，Y. Takeda，AK Mukhopadhyay，S. Garg，SK Bhattacharya，GB Nair和M. Nishibuchi，J. Clin。Microbiol。35：3150-3155，1997）。在这项研究中获得了支持这一假说的证据，该假说仅在最近才出现，并且正在传播到许多国家。在1977年至1998年间孟加拉国一家医院分离的227株菌株中，1996年至1998年之间分离的22株菌株属于新的O3：K6克隆（由血清型， tdh 和 trh定义） 以及AP-PCR配置文件）。从1997年至1998年从台湾，老挝，日本，泰国，韩国和美国的临床来源分离出的O3：K6菌株也显示属于新的O3：K6克隆。通过分析 toxRS 序列也证实了新的O3：K6菌株的克隆性，这已被证明可用于弧菌属成员的系统发育分析。 。新的O3：K6克隆的代表性菌株的 toxRS 序列与1995年之前分离的O3：K6菌株的序列至少在1,346-bp区域的7个碱基位置不同。开发了针对新O3：K6克隆特有的2个碱基位置的新PCR方法。该PCR方法可以清楚地区分属于新O3：K6克隆的所有172菌株与先前分离的其他O3：K6菌株。还通过新的PCR方法检查了除O3：K6以外的属于28个血清型的166个菌株。属于O4：K68和O1：K无法分型血清型的 tdh 阳性和 trh 缺乏菌株在1997年开始在三个国家/地区与国际旅行者隔离积极的结果。这些菌株的AP-PCR谱与新的O3：K6克隆几乎相同，它们的 toxRS 序列与新的O3：K6克隆100％相同。结果表明，这些菌株可能通过改变O：K抗原而不同于新的O3：K6克隆。总之，这项研究为 V史上的首次流行病提供了有力的证据。 parahaemolyticus ，并报道了一种新型的 toxRS 靶向PCR方法，该方法可用于对当前大流行传播相关病例的流行病学调查。

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