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首页> 外文期刊>Journal of Clinical Microbiology >Rapidly Increasing Prevalence of Penicillin-Resistant Streptococcus pneumoniae in Middle Tennessee: a 10-Year Clinical and Molecular Analysis
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Rapidly Increasing Prevalence of Penicillin-Resistant Streptococcus pneumoniae in Middle Tennessee: a 10-Year Clinical and Molecular Analysis

机译：田纳西州中度耐青霉素性肺炎链球菌的流行迅速增加：十年临床和分子分析

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摘要

The clinical and molecular epidemiology of penicillin-resistant Streptococcus pneumoniae and the diagnostic accuracy of a six-primer PCR assay in identifying penicillin resistance were analyzed by using clinical isolates recovered over a 10-year period in middle Tennessee. The prevalence of non-penicillin-susceptible S. pneumoniae isolates (MIC, ≥0.1 μg/ml) increased from 10% in 1990 to 70% in 1999 (P < 0.001). Among S. pneumoniae isolates for which the penicillin MIC was ≥2 μg/ml (highly penicillin-resistant S. pneumoniae [PRSP]), 23 and 5% were resistant to at least three and at least five other antimicrobial classes, respectively. Pulsed-field gel electrophoresis identified 13 unique strain types, with type B accounting for 33% of PRSP isolates. The sensitivity, specificity, and negative and positive predictive values of the PCR assay in detecting PRSP were 99, 100, 99, and 100%, respectively. Penicillin resistance is rapidly increasing among S. pneumoniae isolates in Tennessee. The simultaneous detection of S. pneumoniae and high-level penicillin resistance can be accurately performed with the six-primer PCR assay.
机译：通过使用10株以上回收的临床分离株，分析了耐青霉素的链球菌 肺炎的临床和分子流行病学以及六引物PCR测定法鉴定青霉素耐药性的诊断准确性。年在田纳西州中部。非青霉素易感性 S的患病率。肺炎分离株（MIC，≥0.1μg/ ml）从1990年的10％增加到1999年的70％（ P <0.001）。在 S中。青霉素MIC≥2μg/ ml（高度耐青霉素的肺炎链球菌 [PRSP]），23和5％的肺炎分离株对至少三种和至少三种耐药另外五种抗菌剂。脉冲场凝胶电泳鉴定出13种独特的菌株类型，其中B型占PRSP分离株的33％。 PCR检测PRPR的灵敏度，特异性，阴性和阳性预测值分别为99％，100％，99％和100％。青霉素耐药性在 S中迅速增加。田纳西州的肺炎分离株。同时检测 S。六引物PCR检测可以准确地检测出肺炎和高水平的青霉素耐药性。

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