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首页> 外文期刊>Journal of Clinical Microbiology >Detection, Subgroup Specificity, and Genotype Diversity of Rotavirus Strains in Children with Acute Diarrhea in Paraguay
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Detection, Subgroup Specificity, and Genotype Diversity of Rotavirus Strains in Children with Acute Diarrhea in Paraguay

机译：巴拉圭急性腹泻患儿轮状病毒株的检测，亚组特异性和基因型多样性

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Of a total of 220 stool specimens from children with acute diarrhea, mostly under the age of 3 years, collected in Paraguay between January 1999 and March 2000, 70 (31.8%) were found positive for rotaviruses (RV). Positive samples were characterized by electropherotyping and subgrouping. Sixty-one (87.1%) were classified as group A, subgroup II; one (1.4%) was classified as group A, subgroup I; six (8.6%) were group A, non-I non-II; and two (2.9%) were not tested. RV strains were G and P genotyped by reverse transcription-PCR. The following G types were detected: G4 (34.3%), G1 (21.4%), G2 (1.4%), and G9 (5.7%). Mixtures of human and animal genotypes were detected in 15 (21.4%) samples, and 11 samples (15.7%) were nontypeable. The following P types were detected: P[8] (48.6%), P[4] (1.4%), and P[1] (1.4%). A mixed type was found in 10% of samples, and an unexpectedly high percentage (38.6%) of nontypeable samples was found. The common human G- and P-type combinations P[8], G4 (15.7%) and P[8], G1 (14.2%) were detected. Mixed human and animal genotypes were observed as the following combinations: G4 + G5, G4 + G5 + G10, and G1 + G10 for G types and P[8]-P[1] for P types. The emerging G9 genotype was detected in four samples. These results show for the first time the diversity of RV circulating among children in Paraguay and contribute to the knowledge of this pathogen required to devise strategies to prevent diarrheal illness in this country. The finding of mixed genotypes may indicate interspecies transmission of RV between humans and animals.
机译：在1999年1月至2000年3月间从巴拉圭收集的总共220份粪便标本中，其中大部分是3岁以下的儿童，这些样本主要是3岁以下的急性腹泻，其中70份（31.8％）被发现呈轮状病毒（RV）阳性。阳性样品的特征在于电泳分型和分组。六十一（87.1％）分类为A组，第二亚组;一个（1.4％）被归类为A组，I组; A组为非I非II组，占6个（8.6％）;有两个（2.9％）未进行测试。通过逆转录PCR对RV菌株进行G和P基因分型。检测到以下G类型：G4（34.3％），G1（21.4％），G2（1.4％）和G9（5.7％）。在15个样本（21.4％）中检测到人类和动物基因型的混合物，而11个样本（15.7％）是不可分型的。检测到以下P类型：P [8]（48.6％），P [4]（1.4％）和P [1]（1.4％）。在10％的样本中发现了混合类型，并且发现出乎意料的高百分比（38.6％）的不可分类样本。检测到常见的人类G型和P型组合P [8]，G4（15.7％）和P [8]，G1（14.2％）。观察到人类和动物的混合基因型为以下组合：G型为G4 + G5，G4 + G5 + G10和G1 + G10，P型为P [8] -P [1]。在四个样本中检测到了新兴的G9基因型。这些结果首次显示了巴拉圭儿童中右室循环的多样性，并有助于了解该国制定预防腹泻病策略所需的这种病原体。混合基因型的发现可能表明人与动物之间RV的种间传播。

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