...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Molecular Evidence for Dissemination of Unique Campylobacter jejuni Clones in Cura?ao, Netherlands Antilles
【24h】

Molecular Evidence for Dissemination of Unique Campylobacter jejuni Clones in Cura?ao, Netherlands Antilles

机译：荷属安的列斯库拉索岛独特空肠弯曲菌克隆的分子证据

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Campylobacter jejuni isolates (n = 234) associated with gastroenteritis and the Guillain-Barré syndrome (GBS) in the island of Cura?ao, Netherlands Antilles, and collected from March 1999 to March 2000 were investigated by a range of molecular typing techniques. Data obtained by pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) analysis, multilocus sequence typing (MLST), automated ribotyping, and sequence analysis of the short variable region of the flagellin gene (flaA) were analyzed separately and in combination. Similar groupings were obtained by all methods, with the data obtained by MLST and AFLP analysis exhibiting the highest degree of congruency. MLST identified 29 sequence types, which were assigned to 10 major clonal complexes. PFGE, AFLP analysis, and ribotyping identified 10, 9, and 8 of these clonal groups, respectively; however, these three techniques permitted subdivision of the clonal groups into more different types. Members of seven clonal groups comprising 107 isolates were obtained from November 1999 to February 2000, and no distinguishing characteristics were identified for two GBS-associated strains. The sequence type 41 (ST-41), ST-508, and ST-657 clonal complexes and their corresponding AFLP types have been rare or absent in the Campylobacter data sets described to date. We conclude that several clonal complexes of C. jejuni are associated with human disease in Cura?ao, and some of these have not been reported elsewhere. Furthermore, given the observation that C. jejuni-associated diseases appear to be more severe from November to February, it can be speculated that this may be due to the presence of virulent clones with a limited span of circulation.
机译：空肠弯曲杆菌（ n = 234）与胃肠炎和吉兰-巴雷综合征（GBS）相关，位于荷属安的列斯库拉索岛，于1999年3月收集到2000年3月，通过一系列分子分型技术进行了研究。通过脉冲场凝胶电泳（PFGE），扩增片段长度多态性（AFLP）分析，多位点序列分型（MLST），自动核糖分型以及鞭毛蛋白基因（ flaA ）分别进行了组合分析。通过所有方法获得相似的分组，通过MLST和AFLP分析获得的数据表现出最高的一致性。 MLST确定了29种序列类型，这些序列类型被分配给10种主要的克隆复合体。 PFGE，AFLP分析和核糖分型分别确定了这些克隆组的10、9和8。但是，这三种技术允许将克隆组细分为更多不同的类型。从1999年11月至2000年2月，获得了包括107个分离株的7个克隆组的成员，并且没有发现两个与GBS相关的菌株的区别特征。到目前为止， Campylobacter 数据集中很少或缺少序列类型41（ST-41），ST-508和ST-657的克隆复合物及其对应的AFLP类型。我们得出的结论是 C的几种克隆复合体。空肠与库拉索岛的人类疾病有关，其中一些尚未在其他地方报道。此外，考虑到 C。空肠相关疾病在11月至2月似乎更为严重，可以推测这可能是由于存在有有限传播范围的强力克隆所致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号