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首页> 外文期刊>Journal of Clinical Microbiology >Development of enzyme-labeled oligonucleotide probe for detection of mecA gene in methicillin-resistant Staphylococcus aureus.
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Development of enzyme-labeled oligonucleotide probe for detection of mecA gene in methicillin-resistant Staphylococcus aureus.

机译：用于耐甲氧西林金黄色葡萄球菌mecA基因检测的酶标记寡核苷酸探针的开发。

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摘要

A DNA hybridization method with an enzyme-labeled oligonucleotide probe (mecA-ELONP) was developed to detect the methicillin-resistant gene (mecA) in methicillin-resistant Staphylococcus aureus. For rapid identification, bacterial colonies were transferred from agar plates directly onto nylon membranes. Lysis of cells, denaturation of DNA, and hybridization were performed on the membranes. These procedures required only 3 h for completion. The results obtained by this test closely corresponded with those obtained by determining the MICs of oxacillin against S. aureus. The results of the mecA-ELONP also correlated well with those of a commercially available PCR test. Thus, mecA-ELONP proved to be a reliable and convenient method for the rapid identification of methicillin-resistant S. aureus, which could be useful in clinical microbiology laboratories.
机译：开发了一种用酶标记的寡核苷酸探针（mecA-ELONP）进行DNA杂交的方法，以检测耐甲氧西林的金黄色葡萄球菌中耐甲氧西林的基因（mecA）。为了快速鉴定，将细菌菌落从琼脂平板直接转移到尼龙膜上。在膜上进行细胞裂解，DNA变性和杂交。这些过程仅需要3小时即可完成。通过该测试获得的结果与确定奥沙西林针对金黄色葡萄球菌的MIC所获得的结果非常一致。 mecA-ELONP的结果也与市售PCR测试的结果很好相关。因此，事实证明，mecA-ELONP是快速鉴定耐甲氧西林的金黄色葡萄球菌的可靠且便捷的方法，可在临床微生物学实验室中使用。

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