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首页> 外文期刊>Journal of Clinical Microbiology >Typing of Helicobacter pylori with monoclonal antibodies against Lewis antigens in lipopolysaccharide.
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Typing of Helicobacter pylori with monoclonal antibodies against Lewis antigens in lipopolysaccharide.

机译：用针对脂多糖中Lewis抗原的单克隆抗体对幽门螺杆菌进行分型。

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Recently, it has been shown that the lipopolysaccharide (LPS) O antigen of Helicobacter pylori contains Lewis x (Lex), Lewis y (Ley), or both Lex and Ley antigens. We applied a serotyping method for H. pylori by an enzyme-linked immunosorbent assay with monoclonal antibodies (MAbs) specific for these antigens and the related fucosylated H type 1 (H1) antigen. The selected MAbs recognized the Lex and/or Ley structures in the LPS of H. pylori. The agreement between the results of biochemical compositional analysis and the serological data validated our serotyping system. A total of 152 strains from different geographic origins (The Netherlands, Canada, Poland, Italy, and People's Republic of China) were examined for typeability based on the presence of Lewis antigens. One hundred twenty-nine (84.9%) strains were typeable, and 12 different serotyping patterns were observed; 80.9% of the strains contained Lex and/or Le(y) antigens, and 18.4% reacted with the MAb against the related H1 antigen either alone or in combination with the Lex and/or Ley antigen. Our results show that the Lex and Ley antigens are frequently encountered in the LPS of H. pylori strains from various geographic origins. This typing method is an easy-to-perform technique, which can be used for strain differentiation in epidemiological studies of H. pylori infections.
机译：最近，已经显示幽门螺杆菌的脂多糖（LPS）O抗原包含Lewis x（Lex），Lewis y（Ley）或Lex和Ley抗原。我们通过对这些抗原和相关岩藻糖基化的H型1（H1）抗原具有特异性的单克隆抗体（MAb）的酶联免疫吸附试验，对幽门螺杆菌应用了血清分型方法。所选的单克隆抗体识别幽门螺杆菌LPS中的Lex和/或Ley结构。生化成分分析结果与血清学数据之间的一致性验证了我们的血清分型系统。基于路易斯抗原的存在，共检查了来自不同地理起源（荷兰，加拿大，波兰，意大利和中华人民共和国）的152个菌株的可分型性。 129株（84.9％）可分型，观察到12种不同的血清分型。 80.9％的菌株含有Lex和/或Le（y）抗原，并且18.4％的菌株单独或与Lex和/或Ley抗原结合而与抗相关H1抗原的MAb反应。我们的结果表明，来自不同地理区域的幽门螺杆菌菌株的LPS中经常遇到Lex和Ley抗原。这种分型方法是一种易于执行的技术，可用于幽门螺杆菌感染流行病学研究中的菌株区分。

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