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首页> 外文期刊>Journal of Clinical Microbiology >Production and characterization of monoclonal antibodies specific for the lipopolysaccharide of Escherichia coli O157.
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Production and characterization of monoclonal antibodies specific for the lipopolysaccharide of Escherichia coli O157.

机译：特异于大肠杆菌O157脂多糖的单克隆抗体的生产和表征。

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Identification of the O157 antigen is an essential part of the detection of Escherichia coli O157:H7, which is recognized as a major etiologic agent of hemorrhagic colitis. However, polyclonal antibodies produced against E. coli O157:H7 lipopolysaccharide (LPS) may react with several other bacteria including Brucella abortus, Brucella melitensis, Yersinia enterocolitica O9, Escherichia hermannii, and Stenotrophomonas maltophilia. We produced eight monoclonal antibodies (MAbs) specific for the LPS of E. coli O157. Western blots (immunoblots) of both the phenol phase (smooth) and the aqueous phase (rough) of hot phenol-water-purified LPS indicated that three of the MAbs were specific for the O antigen and five were reactive with the LPS core. The eight MAbs could be further differentiated by their reactivities to Salmonella O30 LPS (group N), which is reported to be identical to the E. coli O157 antigen. All eight MAbs reacted strongly to all of the 64 strains of E. coli O157 tested, which included 47 isolates of O157:H7 and 17 other O157 strains. None of the eight MAbs cross-reacted with any of the 38 other E. coli serotypes tested, which consisted of 29 different O-antigen serotypes, or with 38 strains (22 genera) of non-E. coli gram-negative enteric bacteria.
机译：O157抗原的鉴定是检测大肠杆菌O157：H7的重要部分，该大肠杆菌被认为是出血性结肠炎的主要病因。但是，针对大肠杆菌O157：H7脂多糖（LPS）产生的多克隆抗体可能会与其他几种细菌发生反应，包括流产布鲁氏菌，布鲁氏布鲁氏菌，小肠结肠炎耶尔森氏菌O9，埃尔曼埃希氏菌和嗜麦芽窄食单胞菌。我们生产了八种对大肠杆菌O157的LPS具有特异性的单克隆抗体（MAb）。热酚水纯化的LPS的酚相（平滑）和水相（粗糙）的Western印迹（免疫印迹）表明，三个MAb对O抗原具有特异性，五个MAb与LPS核心具有反应性。八种单克隆抗体可通过它们与沙门氏菌O30 LPS（N组）的反应性进一步区分，据报道与沙门氏菌O157抗原相同。所有八种单克隆抗体均对所测试的64种大肠杆菌O157菌株均产生强烈反应，其中包括47种O157：H7分离株和17种其他O157菌株。八种单克隆抗体中没有一个与其他38种大肠杆菌血清型发生交叉反应，这些血清型由29种不同的O抗原血清型或38种非大肠杆菌菌株（22属）组成。大肠杆菌革兰氏阴性肠细菌。

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