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首页> 外文期刊>Journal of Clinical Microbiology >Clonal Distribution of Methicillin-Resistant Staphylococcus aureus in Poland
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Clonal Distribution of Methicillin-Resistant Staphylococcus aureus in Poland

机译：波兰耐甲氧西林金黄色葡萄球菌的克隆分布

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We report on a study of 158 methicillin-resistantStaphylococcus aureus (MRSA) clinical isolates obtained from 1990 to 1996 in 18 different hospitals in Poland. All isolates were recovered from infection and carriage sites of patients, carriage sites of health care personnel, and hospital environment samples. Fifty-seven MRSA strains described here were studied previously and these were divided into two different clusters according to the degree of heterogeneity of methicillin resistance expression. The aim of this study was to extend the correlation between the two clusters and identify the clonal identities among all isolates by a combination of different methodologies: (i) analysis of mecA polymorphs and Tn554 insertion patterns and (ii) determination of pulsed-field gel electrophoresis patterns of chromosomalSmaI digests. Ninety-seven of 158 strains showed a heterogeneous expression of resistance to methicillin. Among these, 75 (77.3%) were ClaI-mecA type I,ClaI-Tn554 type NH (NH, no homology with transposon Tn554), and pulsed-field gel electrophoresis (PFGE) pattern A (I::NH::A); 10 isolates were III::B::M (10.3%); and the remaining clones included a few or single isolates. The isolates with homogeneous expression of resistance to methicillin (n = 61) were predominantly ClaI-mecA type III (49 of 61 [80.3%]) but had great variability in theirClaI-Tn554 and PFGE patterns. This study confirmed the existence of two main clusters of MRSA in Poland.
机译：我们报告了1990年至1996年在波兰的18家不同医院中获得的158株耐甲氧西林的金黄色葡萄球菌（MRSA）临床分离株的研究。所有分离物均从患者的感染和运送地点，医护人员的运送地点以及医院环境样品中回收。先前研究了这里描述的57个MRSA菌株，根据甲氧西林抗性表达的异质性将它们分为两个不同的簇。这项研究的目的是通过不同方法的组合来扩展两个簇之间的相关性并鉴定所有分离株之间的克隆身份：（i）分析 mecA 多态性和Tn 554 < / em>插入模式和（ii）确定染色体 Sma I消化物的脉冲场凝胶电泳模式。 158个菌株中有97个显示出对甲氧西林的抗性异质表达。其中， Cla I- mecA I型为75，（77.3％）， Cla I-Tn 554 型NH（NH，与转座子Tn 554 没有同源性），以及脉冲场凝胶电泳（PFGE）模式A（I :: NH :: A）; III :: B :: M有10种分离物（10.3％）;其余克隆包括几个或单个分离株。耐甲氧西林的同质表达菌株（ n = 61）主要为III型Cla I- mecA （61/49 [80.3％ ]），但它们的 Cla I-Tn 554 和PFGE模式具有很大的变异性。这项研究证实了波兰MRSA的两个主要集群的存在。

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