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首页> 外文期刊>Journal of Clinical Microbiology >Evidence That the Enterotoxin Gene Can Be Episomal in Clostridium perfringens Isolates Associated with Non-Food-Borne Human Gastrointestinal Diseases
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Evidence That the Enterotoxin Gene Can Be Episomal in Clostridium perfringens Isolates Associated with Non-Food-Borne Human Gastrointestinal Diseases

机译：肠毒素基因在产气荚膜梭状芽孢杆菌分离株中可能是附加型的证据，与非食物源性人类胃肠道疾病有关

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Clostridium perfringens enterotoxin (CPE) is responsible for the diarrheal and cramping symptoms of human C. perfringens type A food poisoning. CPE-producing C. perfringens isolates have also recently been associated with several non-food-borne human gastrointestinal (GI) illnesses, including antibiotic-associated diarrhea and sporadic diarrhea. The current study has used restriction fragment length polymorphism (RFLP) and pulsed-field gel electrophoresis (PFGE) analyses to compare the genotypes of 43 cpe-positive C. perfringensisolates obtained from diverse sources. All North American and European food-poisoning isolates examined in this study were found to carry a chromosomal cpe, while all non-food-borne human GI disease isolates characterized in this study were determined to carry their cpe on an episome. Collectively, these results provide the first evidence that distinct subpopulations ofcpe-positive C. perfringens isolates may be responsible for C. perfringens type A food poisoning versus CPE-associated non-food-borne human GI diseases. If these putative associations are confirmed in additional surveys, cpe RFLP and PFGE genotyping assays may facilitate the differential diagnosis of food-borne versus non-food-borne CPE-associated human GI illnesses and may also be useful epidemiologic tools for identifying reservoirs or transmission mechanisms for the subpopulations ofcpe-positive isolates specifically responsible for CPE-associated food-borne versus non-food-borne human GI diseases.
机译：产气荚膜梭菌肠毒素（CPE）导致人 C的腹泻和痉挛症状。 Perfringens A型食物中毒。产生CPE的 C。产气荚膜菌的分离物最近还与几种非食源性人类胃肠道疾病有关，包括抗生素相关性腹泻和偶发性腹泻。目前的研究使用限制性片段长度多态性（RFLP）和脉冲场凝胶电泳（PFGE）分析来比较43个 cpe 阳性 C的基因型。从不同来源获得的产气荚膜菌的分离物。发现本研究中检查的所有北美和欧洲食物中毒分离株均携带染色体 cpe ，而本研究中表征的所有非食物源性人类胃肠道疾病分离株均携带其在附加体上> cpe 。总的来说，这些结果提供了第一个证据，证明 cpe 阳性 C具有不同的亚群。产气荚膜菌的分离株可能是造成 C的原因。产气荚膜杆菌A型食物中毒与CPE相关的非食源性人类胃肠道疾病相比。如果在其他调查中证实了这些推定的关联，那么 cpe RFLP和PFGE基因分型测定法可能有助于对食源性和非食源性CPE相关的人类胃肠疾病进行鉴别诊断，也可能是有用的流行病学用于确定 cpe 阳性分离株亚群的储库或传播机制的工具，这些分离物专门负责与CPE相关的食源性和非食源性人类胃肠疾病。

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