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首页> 外文期刊>Journal of Clinical Microbiology >Coyotes (Canis latrans) as the Reservoir for a Human Pathogenic Bartonella sp.: Molecular Epidemiology ofBartonella vinsonii subsp. berkhoffii Infection in Coyotes from Central Coastal California
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Coyotes (Canis latrans) as the Reservoir for a Human Pathogenic Bartonella sp.: Molecular Epidemiology ofBartonella vinsonii subsp. berkhoffii Infection in Coyotes from Central Coastal California

机译：土狼（Canis latrans）作为人类致病性巴尔通体sp。的储库：巴尔通体vinsonii亚种的分子流行病学。加州中部沿海的郊狼感染了berkhoffii

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Bartonella vinsonii subsp. berkhoffii was originally isolated from a dog suffering infectious endocarditis and was recently identified as a zoonotic agent causing human endocarditis. Following the coyote bite of a child who developed clinical signs compatible with Bartonella infection in Santa Clara County, Calif., this epidemiological study was conducted. Among 109 coyotes (Canis latrans) from central coastal California, 31 animals (28%) were found to be bacteremic with B. vinsonii subsp.berkhoffii and 83 animals (76%) had B. vinsonii subsp. berkhoffii antibodies. These findings suggest these animals could be the wildlife reservoir of B. vinsonii subsp. berkhoffii. PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the gltA and 16S rRNA genes for these 31 isolates yielded similar profiles that were identical to those of B. vinsonii subsp.berkhoffii. Partial sequencing of the gltA and 16S rRNA genes, respectively, indicated 99.5 and 100% homology between the coyote isolate and B. vinsonii subsp.berkhoffii (ATCC 51672). PCR-RFLP analysis of the 16S-23S intergenic spacer region showed the existence of two different strain profiles, as has been reported in dogs. Six (19%) of 31Bartonella bacteremic coyotes exhibited the strain profile that was identified in the type strain of a canine endocarditis case (B. vinsonii subsp. berkhoffii ATCC 51672). The other 25 bacteremic coyotes were infected with a strain that was similar to the strains isolated from healthy dogs. Based on whole bacterial genome analysis by pulsed-field gel electrophoresis (PFGE) with SmaI restriction endonuclease, there was more diversity in fingerprints for the coyote isolates, which had at least 10 major variants compared to the two variants described for domestic dog isolates from the eastern United States. By PFGE analysis, threeBartonella bacteremic coyotes were infected by a strain identical to the one isolated from three healthy dog carriers. Further studies are necessary to elucidate the mode of transmission of B. vinsonii subsp. berkhoffii, especially to identify potential vectors, and to determine how humans become infected.
机译： Bartonella vinsonii 子亚种。 berkhoffii 最初是从患有传染性心内膜炎的狗中分离出来的，最近被鉴定为引起人心内膜炎的人畜共患病病原体。在加利福尼亚州圣塔克拉拉县，一名儿童的土狼咬伤后出现了与 Bartonella 感染兼容的临床体征，并进行了这项流行病学研究。在加利福尼亚州中部沿海地区的109只土狼（ Canis latrans ）中，有31只动物（28％）被发现具有 B细菌性。 vinsonii 亚种 berkhoffii 和83只动物（76％）患有 B。 vinsonii 子子集。 berkhoffii 抗体。这些发现表明这些动物可能是 B的野生动物保护区。 vinsonii 子子集。 berkhoffii 。对这31个分离株的 gltA 和16S rRNA基因进行PCR限制性片段长度多态性（PCR-RFLP）分析，得出与 B相同的谱图。 vinsonii 亚种 berkhoffii 。 gltA 和16S rRNA基因的部分测序分别表明，土狼分离物和 B有99.5和100％的同源性。 vinsonii 亚种 berkhoffii （ATCC 51672）。如狗中所报道的，对16S-23S基因间隔区的PCR-RFLP分析表明存在两种不同的菌株。 31个 Bartonella 细菌小狼中有6个（19％）表现出在犬心内膜炎病例（ B。vinsonii 亚种 berkhoffii ATCC 51672）。其他25个细菌土狼感染了与从健康犬身上分离的菌株相似的菌株。基于带有 Sma I限制性核酸内切酶的脉冲场凝胶电泳（PFGE）进行的整个细菌基因组分析，土狼分离物的指纹图谱具有更大的多样性，与两种相比，土狼分离物至少具有10个主要变体为来自美国东部的家犬隔离株描述的变体。通过PFGE分析，用与从三种健康狗携带者中分离出的菌株相同的菌株感染了三种 Bartonella 细菌小狼。需要进一步的研究来阐明 B的传播方式。 vinsonii 子子集。 berkhoffii ，尤其是确定潜在的媒介并确定人类如何被感染。

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