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首页> 外文期刊>Journal of Clinical Microbiology >Genomic and Phylogenetic Analysis of Hepatitis C Virus Isolates from Argentine Patients: a Six-Year Retrospective Study
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Genomic and Phylogenetic Analysis of Hepatitis C Virus Isolates from Argentine Patients: a Six-Year Retrospective Study

机译:阿根廷患者丙型肝炎病毒分离株的基因组学和系统发育分析:六年回顾性研究。

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Typing of hepatitis C virus (HCV) isolates from Argentine patients was performed by using different methodologies in a population of 243 patients. HCV subtype was assigned based upon restriction fragment length polymorphism (RFLP). HCV RNA genomes obtained from serum samples were classified as belonging to clade 1 (53.5%), 2 (23.0%), or 3 (8.6%); 14.8% of samples showed HCV mixed infections, more frequently implying different subtypes within the same clade. In addition to RFLP typing, phylogenetic relatedness among sequences from both 5′ untranslated region (n = 50) and nonstructural 5B coding region (n = 15) was established.
机译:对来自阿根廷患者的丙型肝炎病毒(HCV)分离株进行分型,方法是对243名患者使用不同方法。 HCV亚型是根据限制性片段长度多态性(RFLP)分配的。从血清样品中获得的HCV RNA基因组被分为第1类(53.5%),第2类(23.0%)或第3类(8.6%)。 14.8%的样本显示出HCV混合感染,更频繁地暗示着同一进化枝中的不同亚型。除了RFLP分型,还建立了来自5'非翻译区( n = 50)和非结构性5B编码区( n = 15)的序列之间的系统发育关系。

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