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首页> 外文期刊>Journal of Clinical Microbiology >Expression in Escherichia coli of a dominant immunogen of Trypanosoma cruzi recognized by human chagasic sera.
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Expression in Escherichia coli of a dominant immunogen of Trypanosoma cruzi recognized by human chagasic sera.

机译：被人恰加斯氏血清识别的克氏锥虫主要免疫原在大肠杆菌中的表达。

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摘要

A genomic clone expressing a Trypanosoma cruzi antigen in Escherichia coli was identified using human chagasic sera. Chagasic antibodies affinity purified on extracts of this clone recognized a high-molecular-weight protein expressed in all developmental stages of the parasite life cycle, as well as in various T. cruzi strains. The antigen is associated with the cytoskeleton of the parasite and localizes along the attachment region between the flagellum and the cell body. Antibodies to the recombinant antigen were detected in the sera of 115 chagasic patients from different endemic regions, but not in sera of patients with leishmaniasis, T. rangeli infection, or other parasitic diseases. Our data suggest that the presence of antibodies to this antigen may be specifically associated with Chagas' disease.
机译：使用人类chagasic血清鉴定了在大肠杆菌中表达克氏锥虫抗原的基因组克隆。在该克隆的提取物上纯化的亲和力超高效能抗体可识别在寄生虫生命周期的所有发育阶段以及各种克鲁氏梭菌菌株中表达的高分子量蛋白。抗原与寄生虫的细胞骨架相关，并沿着鞭毛和细胞体之间的附着区域定位。在来自不同地方病区的115例chagasic患者的血清中检测到重组抗原的抗体，但未在患有利什曼病，T.rangeli感染或其他寄生虫病的患者的血清中检测到重组抗原。我们的数据表明，针对该抗原的抗体的存在可能与恰加斯氏病特别相关。

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