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首页> 外文期刊>Journal of Clinical Microbiology >Serological follow-up of patients involved in a localized outbreak of leptospirosis.
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Serological follow-up of patients involved in a localized outbreak of leptospirosis.

机译：对局部钩端螺旋体病暴发患者的血清学随访。

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Eighteen patients involved in a localized outbreak of leptospirosis were subjected to a serological follow-up study over a 5-year period. Four distinct sets of sera from all patients and a fifth sample obtained from 10 of them were examined by the microscopic agglutination test (MAT) for demonstration of leptospiral antibodies. The test was carried out by using live leptospires from reference strains of 17 Leptospira interrogans serovars known to occur in Italy. In all cases, the highest titers of agglutinins were recorded against one or more of the three Australis group serovars tested (australis, bratislava, and lora). The highest antibody levels were reached soon after the acute phase of infection in some patients but only after some months in others. Titers then tended to recede with varying rapidity, but titers against the Australis group serovars were still detectable in some patients after 5 years. Coagglutinins against serovars of other serogroups were detected, generally at low levels, in the early sets of sera of most patients, but tended to disappear in the late-set sera. Specific immunoglobulin M (IgM) and IgG against the three Australis group serovars were determined in most serum samples from 16 patients by solid-phase enzyme immunoassay (EIA). In general, EIA titers were considerably lower than MAT titers, but there was a certain patient-to-patient variability in both the IgM/IgG ratio and the evolution and persistence of the two immunoglobulin classes. Since all the evidence indicated that the initial outbreak from a single source, the observed patient-to-patient variability in the progress of both MAT and EIA titers appeared to be attributable to factors inherent in the individual patients. Cross agglutination absorption tests, aimed at retrospectively determining to which of the Australis group serovars the outbreak-specific infecting strain belonged, were performed with six serum samples from different patients. Most absorbed sera seemed to originate from an australis or lora infection, but it was not possible to discriminate conclusively between the two serovars.
机译：涉及局部钩端螺旋体病暴发的18名患者在5年期间接受了血清学随访研究。通过显微镜凝集试验（MAT）检查了所有患者的四组不同的血清以及从其中十组中获得的第五份样品，以证明钩端螺旋体抗体的存在。该测试是通过使用来自意大利已知的17种钩端螺旋体血清型参考菌株的活钩端螺旋体进行的。在所有情况下，记录的凝集素最高滴度都是针对三种被测的极地类群血清中的一种或多种（极地，布拉迪斯拉发和萝拉）。在某些患者中，感染急性期后不久就达到了最高抗体水平，而在另一些患者中则仅在几个月后才达到最高。然后滴度趋于以不同的速度后退，但在5年后的某些患者中仍可检测到极地群血清的滴度。在大多数患者的早期血清中检测到针对其他血清群血清的凝集素，通常水平较低，但在晚期血清中趋于消失。通过固相酶免疫测定法（EIA）在16位患者的大多数血清样本中确定了针对三种极地血清型的特异性免疫球蛋白M（IgM）和IgG。通常，EIA滴度明显低于MAT滴度，但在IgM / IgG比率以及两种免疫球蛋白类别的进化和持久性方面，患者之间存在一定的差异。由于所有证据均表明最初的暴发源于单一来源，因此观察到的MAT和EIA滴度进展中患者之间的差异似乎归因于各个患者的内在因素。交叉凝集吸收试验旨在回顾性地确定来自不同患者的六份血清样本，用于确定暴发特异性感染菌株属于澳大利亚群血清型中的哪一种。吸收的大多数血清似乎源自澳大利亚或劳拉感染，但不可能在两个血清型之间作出明确的区分。

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