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首页> 外文期刊>Journal of Clinical Microbiology >Auxotrophic variants of Pseudomonas aeruginosa are selected from prototrophic wild-type strains in respiratory infections in patients with cystic fibrosis.
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Auxotrophic variants of Pseudomonas aeruginosa are selected from prototrophic wild-type strains in respiratory infections in patients with cystic fibrosis.

机译：铜绿假单胞菌的营养缺陷型选自囊性纤维化患者呼吸道感染的原营养型野生株。

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Twenty-four nutritionally dependent (auxotrophic) Pseudomonas aeruginosa strains were isolated from 20 cystic fibrosis (CF) patients and tested for their amino acid requirements. Two different methods were necessary to identify the nutritional status of all isolates. Methionine was the most common single amino acid required (9 of 24 isolates), followed by leucine and arginine or ornithine. In total, a requirement for 12 different compounds or combination of compounds was demonstrated. Auxotrophic and prototrophic pairs of isolates from the same patient were compared by macrorestriction analysis of DNA in pulsed-field gel electrophoresis. Thirteen of 18 pairs analyzed presented identical restriction fragment length polymorphism profiles following digestion of DNA with XbaI. Three of the remaining pairs showed percentage similarities of 77, 91, and 98%, and the profiles of two pairs could not be compared because of the excessive degradation of their DNA. These results suggest that auxotrophic and prototrophic P. aeruginosa isolates colonizing the same CF patient constitute an isogenic group and raise the possibility that auxotrophs are selected from the prototrophic population during the course of pulmonary infection in CF patients.
机译：从20例囊性纤维化（CF）患者中分离出24种营养相关（营养缺陷型）铜绿假单胞菌菌株，并测试其氨基酸需求。需要两种不同的方法来鉴定所有分离株的营养状况。蛋氨酸是最常见的必需氨基酸（24种分离物中的9种），其次是亮氨酸和精氨酸或鸟氨酸。总共证明了对12种不同化合物或化合物组合的要求。通过在脉冲场凝胶电泳中对DNA进行宏观限制性分析，比较了来自同一患者的营养缺陷型和营养缺陷型对。用XbaI消化DNA后，分析的18对中的13对具有相同的限制性片段长度多态性谱。其余三对显示出77％，91％和98％的百分比相似性，由于两对DNA的过度降解，无法比较两对的概况。这些结果表明，定植在同一CF患者中的营养缺陷型和原养性铜绿假单胞菌分离物构成同基因组，并增加了在CF患者的肺部感染过程中从营养缺陷型群体中选择营养缺陷型的可能性。

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