Transportation of clinical samples and long-term recoverability of pathogens are critical to epidemiological studies, particularly when conditions do not permit immediate processing. This study confirms that Cary-Blair medium (CB) is suitable for the preservation of Salmonella and Shigella isolates for more than 2 weeks at 25, 4, or -70 degrees C. Campylobacter jejuni was not recovered after 2 days of storage in CB at 25 degrees C when an inoculum of 12 x 10(8) cells per ml was used. Lower temperatures supported the recovery of this organism for 6 days. When individual pathogens were preserved with stools in CB and incubated at 25, 4, or -70 degrees C, the Salmonella and Shigella concentrations dropped from 12 x 10(8) cells to 1 x 10(3) or 1 x 10(4) cells per ml within 2 days and then remained stable for the rest of the observation period (15 days). C. jejuni survived preservation with stools for 5 to 9 days. The addition of blood and glycerol to CB improved the recoverability of all enteropathogens, particularly C. jejuni, which was consistently detected for 7 to 9 days at the different preservation temperatures used. When trypticase soy broth-glycerol (freezing medium), with or without blood, was used, there was little or no decrease in the Salmonella and Shigella concentrations during 2 weeks of preservation with stools at -70 degrees C. C. jejuni demonstrated a relatively sustained high concentration in Trypticase soy broth-glycerol with 5% blood. The use of defibrinated, laked sheep blood as a long-term freezing medium supported the recovery of low concentrations of Salmonella and Shigella spp. (10(2) to 10(3) cells per ml) for more than 14 weeks. Recovery of C. jejuni was consistent for 7 weeks when an initial concentration of 10(6) cells per ml present in stools. Laked blood provided a simple, sterile, and inexpensive medium for the preservation of individual isolates and clinical samples.
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机译：临床样品的运输和病原体的长期可回收性对于流行病学研究至关重要，特别是在条件不允许立即处理的情况下。这项研究证实，Cary-Blair培养基（CB）适用于沙门氏菌和志贺氏菌分离株在25、4或-70摄氏度下保存2周以上。空肠弯曲菌在CB中保存2天后未恢复当使用每毫升12 x 10（8）个细胞的接种体时，温度为25摄氏度。较低的温度支持该生物体恢复了6天。当个别病原体在粪便中保存在CB中并在25、4或-70摄氏度下孵育时，沙门氏菌和志贺氏菌的浓度从12 x 10（8）细胞降至1 x 10（3）或1 x 10（4）在2天之内每毫升细胞数，然后在整个观察期（15天）保持稳定。空肠弯曲杆菌在粪便保存下存活5至9天。在CB中添加血液和甘油可改善所有肠道病原体（特别是空肠弯曲杆菌）的可恢复性，在使用的不同保存温度下可连续检测7至9天。当使用胰酶解酶大豆肉汤甘油（冷冻培养基），有或没有血液时，沙门氏菌和志贺氏菌的浓度在保存两周期间几乎没有或没有下降，空肠在-70度CC空肠中表现出相对持续的高浓度含5％血液的胰蛋白酶酶大豆肉汤甘油中。使用去纤维化的湖血绵羊血作为长期冷冻培养基可支持低浓度沙门氏菌和志贺氏菌的回收。 （每毫升10（2）至10（3）个细胞）超过14周。当粪便中初始浓度为每毫升10（6）个细胞时，空肠弯曲杆菌的恢复持续7周。淀积的血液提供了一种简单，无菌且廉价的培养基，用于保存单个分离株和临床样品。
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