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首页> 外文期刊>Journal of Clinical Microbiology >Isolation of human immunodeficiency virus type 1 (HIV-1) RNA from feces by a simple method and difference between HIV-1 subpopulations in feces and serum.
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Isolation of human immunodeficiency virus type 1 (HIV-1) RNA from feces by a simple method and difference between HIV-1 subpopulations in feces and serum.

机译：通过一种简单的方法从粪便中分离人类免疫缺陷病毒1型（HIV-1）RNA，以及粪便和血清中HIV-1亚群之间的差异。

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摘要

A simple method for the isolation and subsequent detection of human immunodeficiency virus type 1 (HIV-1) RNA from feces is described. Viral RNA was isolated by the method developed by Boom et al. (R. Boom, C.J.A. Sol, M.M.M. Salimans, C.L. Jansen, P.M.E. Wertheim-van Dillen, and J. van der Noordaa, J. Clin. Microbiol. 28:495-503, 1990), which was adapted for feces. HIV-1 RNA was detected by reverse transcription (RT) followed by a nested PCR encompassing the V3 region. Reconstruction experiments revealed that the efficiencies of the extraction technique and the subsequent RT-PCR were not considerably affected by the varied composition of feces. The method was applied on fecal specimens from 18 HIV-1-infected individuals, among which were samples that had been stored for 9 years. It appeared that HIV-1 RNA was detectable in the feces of 12 persons (67%). Viral RNA was present in the feces of persons who fulfilled the criteria for CDC class II and CDC class III HIV infection as well as in patients who were diagnosed with AIDS (CDC class IV). Direct sequencing of amplimers obtained from paired fecal and serum specimens showed that differences in sequence heterogeneity existed. In one patient a remarkable difference in the HIV-1 sequences between isolates from feces and serum was observed. In conclusion, HIV-1 RNA is frequently present in the feces of HIV-1-infected individuals, and in some cases the HIV-1 subpopulation in feces differs from the HIV-1 subpopulation in serum.
机译：描述了一种从粪便中分离和随后检测人类免疫缺陷病毒1型（HIV-1）RNA的简单方法。通过Boom等人开发的方法分离病毒RNA。 （R.Boom，C.J.A. Sol，M.M.M. Salimans，C.L. Jansen，P.M.E. Wertheim-van Dillen，and J.van der Noordaa，J.Clin.Microbiol.28：495-503，1990），适用于粪便。通过逆转录（RT），然后是包含V3区的嵌套PCR，检测到HIV-1 RNA。重建实验表明，提取技术和后续RT-PCR的效率不受粪便成分变化的影响。该方法应用于18名HIV-1感染者的粪便样本，其中样本已保存9年。似乎在12个人（67％）的粪便中可检测到HIV-1 RNA。在符合CDC II类和CDC III类HIV感染标准的人以及被诊断患有AIDS的患者（CDC IV类）的粪便中存在病毒RNA。直接从配对的粪便和血清标本中获得的扩增子测序表明存在序列异质性差异。在一名患者中，观察到粪便和血清分离株之间的HIV-1序列存在显着差异。总之，HIV-1 RNA经常出现在感染HIV-1的个体的粪便中，在某些情况下，粪便中的HIV-1亚群与血清中的HIV-1亚群有所不同。

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