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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of monoclonal antibodies to the hemagglutinin-esterase glycoprotein of a bovine coronavirus associated with winter dysentery and cross-reactivity to field isolates.
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Characterization of monoclonal antibodies to the hemagglutinin-esterase glycoprotein of a bovine coronavirus associated with winter dysentery and cross-reactivity to field isolates.

机译：牛冠状病毒血凝素-酯酶糖蛋白单克隆抗体的表征，与冬季痢疾和与野外分离株的交叉反应有关。

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Seven hybridoma cell lines producing monoclonal antibodies (MAbs) to the hemagglutinin-esterase (HE) glycoprotein of bovine coronavirus (BCV) were obtained from BALB/c mice that were immunized with an enriched peplomeric fraction of the winter dysentery (WD)-associated strain BCQ.2590. The specificities of these MAbs to either the dimeric (140-kDa) or the monomeric (65-kDa) form of the HE glycoprotein were determined by Western immunoblotting experiments with purified virus and immunoprecipitation tests with [35S]methionine-labelled infected cell extracts. Four of these anti-HE MAbs inhibited the hemagglutinating activity of the virus and three weakly neutralized its infectivity in vitro. In addition, competition binding assays allowed for the definition of two independent antigenic domains (domains A and D) and two overlapping antigenic domains (domains B and C) for the HE glycoprotein of the WD-associated strain; epitopes located within antigenic domain A were not associated with hemagglutination inhibition (HAI) and virus neutralization activities. In HAI tests, the four anti-HA MAbs defined two distinct antigenic subgroups among 24 BCV field isolates that have been associated with either typical outbreaks of WD or neonatal calf diarrhea (NCD) in Quebec dairy herds from 1986 to 1996. The Quebec WD-associated strains of BCV, as well as some of the NCD-associated strains isolated since 1991, fell within a subgroup distinct from that of the prototype Mebus strain.
机译：从BALB / c小鼠中获得了七种产生针对牛冠状病毒（BCV）血凝素酯酶（HE）糖蛋白的单克隆抗体（MAb）的杂交瘤细胞系，并用与冬季痢疾（WD）相关的菌株的富二倍体部分免疫了BALB / c小鼠BCQ.2590。这些MAb对HE糖蛋白的二聚体（140-kDa）或单体（65-kDa）形式的特异性通过纯化病毒的Western免疫印迹实验和用[35S]蛋氨酸标记的感染细胞提取物的免疫沉淀试验确定。这些抗HE MAb中有4种抑制病毒的血凝活性，而3种在体外弱化了其感染性。另外，竞争结合测定允许为WD相关菌株的HE糖蛋白定义两个独立的抗原结构域（结构域A和D）和两个重叠的抗原结构域（结构域B和C）。位于抗原域A内的抗原决定簇与血凝抑制（HAI）和病毒中和活​​性无关。在HAI测试中，四种抗HA MAb定义了24个BCV分离株中的两个不同的抗原亚组，这些分离株与1986年至1996年在魁北克奶牛群中典型的WD爆发或新生儿小牛腹泻（NCD）有关。Quebec WD- BCV的相关菌株，以及自1991年以来分离出的一些与NCD相关的菌株，都属于与原型Mebus菌株不同的亚组。

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