Development of a PCR assay for rapid diagnosis of Mycobacterium ulcerans infection.

B C Ross; L Marino; F Oppedisano; R Edwards; R M Robins-Browne; P D Johnson

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Development of a PCR assay for rapid diagnosis of Mycobacterium ulcerans infection.

机译：快速诊断溃疡分枝杆菌感染的PCR检测方法的开发。

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The diagnosis of Mycobacterium ulcerans infection is hampered by the slow growth of the bacterium in culture, resulting in a delay of several months before a specific diagnosis can be obtained. In addition, M. ulcerans cannot be isolated from water even when there is convincing epidemiological evidence implicating this as the source of infection. The aim of the present study was to develop a PCR assay to circumvent the problems of delayed diagnosis and insensitivity of standard bacterial culture for M. ulcerans. For the PCR, we isolated an M. ulcerans-specific DNA fragment, 1,109 bp long, which is repeated at least 50 times throughout the genome. Use of this sequence as a target for PCR allowed us to detect as few as 2 molecules of genomic DNA in vitro. The PCR was used to detect M. ulcerans DNA in fresh tissue and paraffin-embedded sections from all seven patients with culture-confirmed cases of infection.

机译：细菌分枝杆菌在培养中的缓慢生长阻碍了溃疡分枝杆菌感染的诊断，导致延迟数月才能获得特定的诊断。此外，即使有令人信服的流行病学证据表明它是感染源，也无法从水中分离出溃疡分枝杆菌。本研究的目的是开发一种PCR检测方法，以解决溃疡分枝杆菌的诊断延迟和标准细菌培养不敏感的问题。对于PCR，我们分离出了溃疡分枝杆菌特异性DNA片段，长1,109 bp，在整个基因组中重复至少50次。使用该序列作为PCR的靶标使我们能够在体外检测到2个分子的基因组DNA。 PCR用于检测所有7例经培养确诊的感染病例的新鲜组织和石蜡包埋切片中的溃疡分枝杆菌DNA。

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《Journal of Clinical Microbiology》 |1997年第7期|共5页
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B C Ross; L Marino; F Oppedisano; R Edwards; R M Robins-Browne; P D Johnson;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Sensitivity of PCR Targeting the IS2404 Insertion Sequence of Mycobacterium ulcerans in an Assay Using Punch Biopsy Specimens for Diagnosis of Buruli Ulcer [J] . R. Phillips, C. Horsfield, S. Kuijper, Journal of Clinical Microbiology . 2005,第8期

机译：PCR检测靶向溃疡分枝杆菌的IS2404插入序列的敏感性，使用穿孔活检标本诊断布鲁氏溃疡
2. Development and Application of Two Multiplex Real-Time PCR Assays for the Detection of Mycobacterium ulcerans in Clinical and Environmental Samples [J] . Janet A. M. Fyfe, Caroline J. Lavender, Paul D. R. Johnson, Applied and Environmental Microbiology . 2007,第15期

机译：检测临床和环境样品中溃疡分枝杆菌的两种多重实时荧光定量PCR检测方法的开发和应用
3. Development and Application of Real-Time PCR Assay for Quantification of Mycobacterium ulcerans DNA [J] . S. Rondini, E. Mensah-Quainoo, H. Troll, Journal of Clinical Microbiology . 2003,第9期

机译：实时PCR检测定量分支杆菌DNA的开发与应用
4. DEVELOPMENT AND VALIDATION OF A DIRECT REAL-TIME PCR ASSAY FOR MYCOBACTERIUM BOVIS AND IMPLEMENTATION INTO THE UNITED STATES NATIONAL SURVEILLANCE PROGRAM [C] . Philip Dykema, Kevin Stokes, Nadine Beckwith, Annual Meeting of the United^States^Animal^Health^Association. . 2016

机译：基因分枝杆菌直接实时PCR测定的开发与验证，并在美国国家监测计划中的实施
5. Development of better methods for the laboratory diagnosis of Mycobacterium ulcerans disease (Buruli ulcer) and search for environmental reservoirs of Mycobacterium ulcerans. [D] . Eddyani, Miriam. 2009

机译：开发更好的实验室诊断方法，用于溃疡分枝杆菌病（Buruli溃疡）和寻找环境分支杆菌。
6. Development of a PCR assay for rapid diagnosis of Mycobacterium ulcerans infection. [O] . B C Ross, L Marino, F Oppedisano, 1997

机译：快速诊断溃疡分枝杆菌感染的PCR检测方法的开发。
7. Sensitivity of PCR Targeting the IS2404 Insertion Sequence of Mycobacterium ulcerans in an Assay Using Punch Biopsy Specimens for Diagnosis of Buruli Ulcer [O] . Phillips, R., Horsfield, C., Kuijper, S., 2005

机译：PCR检测针对溃疡分枝杆菌IS2404插入序列的PCR的敏感性，使用穿孔活检标本诊断布鲁氏溃疡
8. Development of the Biological Immuno-Magnetic Assay System for Rapid Diagnosis from Clinical Samples [R] . Calomiris, J. J. 1998

机译：临床样品快速诊断生物免疫磁分析系统的研制

Development of a PCR assay for rapid diagnosis of Mycobacterium ulcerans infection.

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