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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of urinary Escherichia coli O75 strains.
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Characterization of urinary Escherichia coli O75 strains.

机译：尿大肠杆菌O75菌株的表征。

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Forty-four Escherichia coli O75 strains from patients with urinary tract infections were characterized by a variety of methods to obtain evidence of their clonal distribution and uropathogenic properties. By K and H antigen typing, the strains were divided into the following serotypes: O75:K5:H- (18 strains), O75:K95:H- (10 strains), O75:K95:H5 (7 strains), O75:K100:H5 (4 strains), and O75:K-:H55 (5 strains). Generally, biotyping proved to be of no discriminative value. With two exceptions the strains were found to be sensitive to the bactericidal effect of normal human serum. As shown by multilocus enzyme electrophoresis, the whole-cell protein profile (WCPP), and the patterns of the outer membrane proteins and lipopolysaccharides, all but the five O75:H55 strains were genetically closely related to each other and could be classified into one clonal group. The O75:K-:H55 strains proved to be quite different and lacked type 1 fimbriae. All 17 K95 (H-, H5) strains produced hemolysin and P fimbriae. Five of the O75:K5:H- strains were different from the other K5 strains by showing hemagglutinating properties, on the basis of the presence of the OX adhesin. The last two groups are suggested to be uropathogenic and are proposed to represent separate clonal groups or subgroups.
机译：通过多种方法对来自尿路感染患者的44株O75菌株进行了表征，以获取其克隆分布和尿毒症特性的证据。通过K和H抗原分型，将菌株分为以下血清型：O75：K5：H-（18株），O75：K95：H-（10株），O75：K95：H5（7株），O75： K100：H5（4株）和O75：K-：H55（5株）。通常，生物分型被证明没有区别价值。除了两个例外，发现该菌株对正常人血清的杀菌作用敏感。如多位酶电泳，全细胞蛋白谱（WCPP）以及外膜蛋白和脂多糖的模式所示，除5株O75：H55菌株外，它们在遗传上都密切相关，可以分为一个克隆组。 O75：K-：H55菌株证明完全不同，并且缺少1型菌毛。所有17个K95（H-，H5）菌株均产生溶血素和P菌毛。基于OX黏附素的存在，O75：K5：H-菌株中有五个与其他K5菌株表现出血凝特性不同。最后两个组被认为是泌尿生殖系统疾病，被提议代表不同的克隆组或亚组。

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