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首页> 外文期刊>Journal of Clinical Microbiology >Enzyme-linked immunosorbent assay to measure antibodies to purified heat-labile enterotoxins from human and porcine strains of Escherichia coli and to cholera toxin: application in serodiagnosis and seroepidemiology.
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Enzyme-linked immunosorbent assay to measure antibodies to purified heat-labile enterotoxins from human and porcine strains of Escherichia coli and to cholera toxin: application in serodiagnosis and seroepidemiology.

机译：酶联免疫吸附法可检测人和猪大肠杆菌中纯化的对热不稳定的肠毒素和霍乱毒素的抗体：在血清诊断和血清流行病学中的应用。

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Serum immunoglobulin G antibodies to purified heat-labile enterotoxin (LT) from human (LTh) and porcine (LTp) Escherichia coli strains and cholera enterotoxin (CT) were measured by an enzyme-linked immunosorbent assay. Sera from patients with LTh E. coli infection showed a prominent response with LTh, an intermediate response with LTp, and a meager response with CT. Of 47 persons with clinical LTh-producing E. coli (herein shortened to LTh E. coli) infections, significant rises in antitoxin were detected against LTh in 36 (77%), against LTp in 30 (64%), and against CT in only 13 (28%) patients; seroconversions also occurred in 11 of 14 (79%) patients with subclinical LTh E. coli infections. In North Americans with experimental LTh E. coli infection, anti-Lth did not remain at high levels for more than 3 months. Persons with cholera manifested antitoxin responses that were similarly potent against all three toxin antigens; in fact, net optical density values were often slightly higher against LTh than against CT. The ratio of CT/LTh ELISA net optical density in convalescent sera proved to be a sensitive means to differentiate LT E. coli from cholera infection. All 11 cholera patients tested had CT/LTh ratios of greater than 0.70, whereas in only 1 of 47 LTh E. coli infections did the ratio exceed that value (it was 0.71) (P less than 0.0000000001). In single serum specimens, a net optical density of greater than or equal to 0.30 against LTh was shown to be a useful cutoff in screening sera for recent LTh E. coli or past cholera infection. The CT/LTh ratio was then used to differentiate definitively. Sera from healthy 3- to 5-year -olds and 15- to 19-year-olds in Maryland, Chile, and Bangladesh were tested against LTh and CT. The serological results fit known epidemiological observations. (i) LTh infections are rare in the United States (only 2 of 60 sera had LTh net optical density values of >/= 0.30. (ii) In contrast, evidence of recent LTh E. coli infections was very common in Chilean (69%) and Bangladeshi (57%) 3- to 5-year-olds and not uncommon in 15- to 19-year-olds (38 and 31%, respectively) in those countries. (iii) Only Bangladeshi sera showed serological evidence of cholera infections (CT/LTh ratios of > 0.70). The immunoglobulin G enzyme-linked immunosorbent assay measuring antibodies to purified LTh and CT represents a practical and effective tool for the serological study of LTh E. coli and cholera diarrheal infections.
机译：通过酶联免疫吸附测定法测定了针对来自人（LTh）和猪（LTp）的大肠杆菌菌株和霍乱肠毒素（CT）的纯化的热不稳定肠毒素（LT）的血清免疫球蛋白G抗体。 LTh大肠杆菌感染患者的血清对LTh的反应显着，对LTp的反应中等，对CT的反应较差。在47例临床上会产生LTh的大肠杆菌（以下简称LTh大肠杆菌）感染的人中，针对LTh的抗毒素显着上升（77％），针对Lp的抗毒素显着上升（30％）（64％），针对CT的抗毒素上升。仅13位（28％）患者;在亚临床LTh大肠杆菌感染的14例患者中，有11例（79％）发生了血清转化。在患有实验性LTh大肠杆菌感染的北美人中，抗Lth不会在3个月以上的时间内保持高水平。霍乱患者表现出的抗毒素反应对所有三种毒素抗原同样有效。实际上，对LTh的净光学密度值通常比对CT略高。恢复期血清中CT / LTh ELISA净光密度之比被证明是区分LT大肠杆菌与霍乱感染的灵敏手段。测试的所有11名霍乱患者的CT / LTh比率均大于0.70，而在47例LTh大肠杆菌感染中，只有1例的比率超过了该值（0.71）（P小于0.0000000001）。在单个血清样本中，针对LTh的净光密度大于或等于0.30被证明是筛选血清以检测近期LTh大肠杆菌或过去霍乱感染的有用分界点。然后使用CT / LTh比值进行区分。在马里兰州，智利和孟加拉国，对健康的3至5岁儿童和15至19岁儿童的血清进行了LTh和CT测试。血清学结果符合已知的流行病学观察结果。 （i）LTh感染在美国很少见（60个血清中只有2个的LTh净光密度值> / = 0.30。（ii）相反，最近LTh大肠杆菌感染的证据在智利非常普遍（69 （％）和孟加拉国（57％）的3至5岁儿童，在这些国家的15至19岁儿童中（分别为38％和31％）并不罕见（iii）只有孟加拉国血清显示出血清学证据霍乱感染（CT / LTh比> 0.70）免疫球蛋白G酶联免疫吸附法可检测纯化的LTh和CT的抗体，是对LTh大肠杆菌和霍乱腹泻感染进行血清学研究的实用有效工具。

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