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首页> 外文期刊>Journal of Clinical Microbiology >Identification of group B streptococcal antigen with lectin-bound polystyrene particles.
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Identification of group B streptococcal antigen with lectin-bound polystyrene particles.

机译：用凝集素结合的聚苯乙烯颗粒鉴定B组链球菌抗原。

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摘要

The lectin of the tomato, Lycopersicon esculentum, or of the potato, Solanum tuberosum, can be passively coupled to amide-modified polystyrene spheres to be used as a detection reagent for the specific identification of group B streptococcal cultures grown in selective or nonselective Todd-Hewitt broth for 5 and 4 h, respectively. Agglutination occurred when the lectin reagents were allowed to react with either the cell suspension, clarified broth, or antigen extracts from group B streptococci grown in Todd-Hewitt broth. No agglutination occurred when these lectins were allowed to react with strains of serogroup A, C, D, F, or G streptococci. False-negative agglutination responses may occur with certain serotype of group B streptococci grown on Columbia sheep blood agar. A 20-min staining time permitted the specific labeling of fixed smears of group B streptococci with fluorescein-conjugated Lycopersicon lectin. The lectin from the solanaceous plant Datura stramonium did not agglutinate group B streptococci or other clinically significant streptococcal serogroups.
机译：番茄，番茄或马铃薯的凝集素可以被动偶联到酰胺修饰的聚苯乙烯球上，用作检测试剂，以特异性鉴定在选择性或非选择性托德氏菌中生长的B组链球菌培养物。翰威特肉汤分别持续5和4小时。当凝集素试剂与细胞悬浮液，澄清的肉汤或在Todd-Hewitt肉汤中生长的B组链球菌的抗原提取物反应时，发生凝集。当这些凝集素与血清群A，C，D，F或G链球菌发生反应时，没有发生凝集反应。在哥伦比亚绵羊血琼脂上生长的某些血清型的B组链球菌可能发生假阴性凝集反应。 20分钟的染色时间允许用荧光素结合的Lycopersicon凝集素特异性标记B组链球菌的固定涂片。茄科植物曼陀罗（Datura stramonium）的凝集素未凝集B组链球菌或其他具有临床意义的链球菌血清群。

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