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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of different fixatives and treatments for immunohistochemical demonstration of Coxiella burnetti in paraffin-embedded tissues.
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Evaluation of different fixatives and treatments for immunohistochemical demonstration of Coxiella burnetti in paraffin-embedded tissues.

机译：在石蜡包埋的组织中评估不同固定剂和治疗方法的免疫组织化学证明柯氏杆菌。

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摘要

Various fixatives and treatments such as acetone, methanol, Bouin fixative, modified Bouin fixative, 10% Formalin, modified methacarn, periodate-lysine-paraformaldehyde, acetone-methyl benzoate-xylene, and EDTA were evaluated for their effect on the immunoreactivity of Coxiella burnetii in paraffin-embedded tissues by using the avidin-biotin-peroxidase complex and the peroxidase-antiperoxidase procedure. C. burnetii antigen was shown to be present in liver, spleen, and uterus tissues of experimentally infected mice by all methods of fixation and treatment. A positive immunoreaction was seen in cytoplasmic vacuoles of macrophages, as extracellular rod-shaped organisms, and as residual particulate extra- and intracellular debris. Immunoreactivity and cellular preservation, however, varied substantially with the individual fixatives. Optimal immunostaining of C. burnetii was achieved by EDTA treatment and Bouin and acetone fixation. The avidin-biotin-peroxidase technique proved to be slightly more sensitive than the peroxidase-antiperoxidase procedure when primary antibody dilution was used as the criterion for sensitivity.
机译：评估了丙酮，甲醇，Bouin固定剂，修饰的Bouin固定剂，10％福尔马林，修饰的美沙芬，高碘酸-赖氨酸-多聚甲醛，丙酮-苯甲酸甲酯-二甲苯和EDTA等各种固定剂和处理剂对烧伤柯氏杆菌免疫反应性的影响。通过使用抗生物素蛋白-生物素-过氧化物酶复合物和过氧化物酶-抗过氧化物酶程序在石蜡包埋的组织中分离。通过所有固定和治疗方法，证明伯氏梭状芽胞杆菌抗原存在于实验感染小鼠的肝脏，脾脏和子宫组织中。在巨噬细胞的细胞质液泡中，作为细胞外棒状生物以及残留的颗粒外和细胞内碎片中观察到阳性免疫反应。但是，免疫反应性和细胞保存随各个固定剂的不同而有很大差异。 EDTA处理以及Bouin和丙酮固定可实现最佳的Burnetii梭菌免疫染色。当将一抗稀释度用作敏感性标准时，抗生物素蛋白-生物素-过氧化物酶技术被证明比过氧化物酶-抗过氧化物酶方法稍敏感。

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