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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of nine commercial immunoassays for the detection of rotavirus in fecal specimens.
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Comparison of nine commercial immunoassays for the detection of rotavirus in fecal specimens.

机译：粪便标本中轮状病毒检测的九种商业免疫检测方法的比较。

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One hundred fecal specimens obtained from patients with acute gastroenteritis were tested for rotavirus with nine commercial immunoassays to evaluate the sensitivity, specificity, predictive value, and diagnostic accuracy of these assays. Kits evaluated included two monoclonal antibody-based enzyme immunoassays (EIAs) (Rotaclone and Pathfinder Rotavirus), three polyclonal antibody-based EIAs (Rotavirus Immunoassay, Rotazyme II, and Wellcozyme Rotavirus), and four latex agglutination assays (Rotastat, Virogen Rotatest, Meritec-Rotavirus, and The Wellcome Latex Test). Thirty-eight of the 100 specimens were found to contain rotavirus by a reference microplate EIA. The accuracy of the reference assay was determined by RNA electrophoresis and a blocking assay on discordant specimens. The two monoclonal antibody EIAs had superior sensitivities (100%) and identified two positive specimens which were negative by the reference method but positive by the blocking assay. Among the polyclonal EIAs, all had sensitivities of greater than 90%, but specificities were variable; Rotazyme II, with a specificity of 50%, showed considerable discrepancy from other polyclonal EIAs. The latex tests had sensitivities ranging from 70 to 90% and specificities of 80 to 100%. Latex agglutination tests were more rapid than EIAs and did not require expensive equipment. The final choice of assay system will depend on the cost, speed, and accuracy requirements of the clinical laboratory.
机译：用九种商业免疫测定法对从急性胃肠炎患者获得的100份粪便标本进行轮状病毒测试，以评估这些测定法的敏感性，特异性，预测价值和诊断准确性。评估的试剂盒包括两个基于单克隆抗体的酶免疫测定（EIA）（罗塔克隆和Pathfinder轮状病毒），三个基于多克隆抗体的EIA（轮状病毒免疫测定，Rotazyme II和Wellcozyme轮状病毒），以及四个乳胶凝集测定（Rotastat，Virogen Rotatest，Meritec -轮状病毒和惠康乳胶测试）。通过参考微孔板EIA，发现100个样本中有38个含有轮状病毒。参比测定的准确性通过RNA电泳和不连贯标本的封闭测定来确定。两种单克隆抗体EIA具有较高的灵敏度（100％），并鉴定出两个阳性标本，这些标本通过参考方法为阴性，但通过封闭分析为阳性。在多克隆EIA中，所有EIA的敏感性均高于90％，但特异性是可变的。 Rotazyme II的特异性为50％，显示出与其他多克隆EIA的显着差异。乳胶测试的敏感性范围为70％至90％，特异性为80％至100％。乳胶凝集测试比EIA更快，并且不需要昂贵的设备。分析系统的最终选择将取决于临床实验室的成本，速度和准确性要求。

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