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首页> 外文期刊>Journal of Clinical Microbiology >Isolation of three distinct parasite populations expressing different circumsporozoite proteins from the Philippine strain of Plasmodium knowlesi.
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Isolation of three distinct parasite populations expressing different circumsporozoite proteins from the Philippine strain of Plasmodium knowlesi.

机译：从菲律宾恶性疟原虫菌株分离表达不同环子孢子蛋白的三个不同的寄生虫种群。

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Approximately 50% of the sporozoites of the Philippine (P) strain of Plasmodium knowlesi were found to react with monoclonal antibodies, produced against sporozoites of the H strain of P. knowlesi, by indirect immunofluorescence. Two of these anti-H strain monoclonal antibodies were used with the P-strain sporozoites in a neutralization assay. They selectively abolished the infectivity of part of the sporozoite population, and the remainder was designated the P2 strain. Monoclonal antibodies produced against sporozoites of the P2 strain identified two different sets of circumsporozoite proteins by Western blot analysis of an extract of these parasites, indicating the presence of two different populations of sporozoites. These two populations were separated by neutralization assays with monoclonal antibodies that recognized the two different sets of circumsporozoite proteins. The corresponding two strains were designated P3A and P3B. By Western blot analysis, two proteins with apparent molecular weights of 41,000 and 51,000 were identified in extracts of sporozoites of the P3A strain, and two proteins with molecular weights of 38,000 and 47,000 were identified in extracts of the P3B sporozoites.
机译：发现通过间接免疫荧光，约50％的知识型疟原虫的菲律宾（P）菌株的子孢子与单克隆抗体反应，所述单克隆抗体针对知识型疟原虫的H菌株的子孢子产生。在中和测定中，将这些抗H株单克隆抗体中的两种与P株子孢子一起使用。他们有选择地取消了部分子孢子种群的传染性，其余被称为P2菌株。通过对这些寄生虫的提取物进行蛋白质印迹分析，确定了针对P2菌株子孢子的单克隆抗体鉴定了两组不同的环子孢子蛋白，表明存在两种不同的子孢子种群。通过使用单克隆抗体的中和测定法分离了这两个种群，所述单克隆抗体识别两组不同的环子孢子蛋白。相应的两个菌株被命名为P3A和P3B。通过蛋白质印迹分析，在P3A菌株的子孢子提取物中鉴定出两种分子量分别为41,000和51,000的蛋白质，并且在P3B子孢子的提取物中鉴定出两种分子量分别为38,000和47,000的蛋白质。

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