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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of two single-plate incubation systems and the anaerobic chamber for the cultivation of anaerobic bacteria.
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Evaluation of two single-plate incubation systems and the anaerobic chamber for the cultivation of anaerobic bacteria.

机译：评估两个单板温育系统和用于培养厌氧细菌的厌氧室。

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Three systems that are available for the incubation of anaerobic organisms were evaluated to assess their ability to support the growth of 25 anaerobic stock strains and to successfully recover anaerobic bacteria from clinical specimens. These were the anaerobic chamber, the Anaerobic Pouch System Catalyst-Free (Difco Laboratories, Detroit, Mich.), and the Bio-Bag Environmental Chamber Type A (Marion Scientific, Div. Marion Laboratories, Inc., Kansas City, Mo.). Three study centers were involved, the Wadsworth Anaerobe Laboratory (Los Angeles, Calif.), the Good Samaritan Hospital (San Jose, Calif.), and the Massachusetts General Hospital (Boston). A total of 171 anaerobic organisms were isolated from 49 clinical specimens that were cultured at the three test centers. Of these, 169 (99%) were recovered from media that were incubated in the anaerobic chamber, 163 (95%) were recovered from the Anaerobic Pouch, and 147 (86%) were recovered from the Bio-Bag. A similar trend was seen with the stock strains, in which the anaerobic chamber often supported better growth of the organisms than did either of the bag systems.
机译：评估了可用于厌氧菌培养的三个系统，以评估其支持25种厌氧菌种生长以及成功从临床标本中回收厌氧菌的能力。这些是厌氧室，厌氧袋系统无催化剂（密歇根州底特律的Difco实验室）和A型生物袋环境室（密苏里州堪萨斯市的Marion Scientific，Div。Marion Laboratories，Inc.）。 。三个研究中心参与其中，Wadsworth Anaerobe实验室（加利福尼亚州洛杉矶），Good Samaritan医院（加利福尼亚州圣何塞）和马萨诸塞州综合医院（波士顿）。从在三个测试中心培养的49个临床标本中总共分离出171个厌氧菌。其中，从在厌氧室中培养的培养基中回收了169（99％），从厌氧袋中回收了163（95％），从Bio-Bag中回收了147（86％）。菌种也有类似的趋势，其中厌氧室通常比两种制袋系统都支持更好的生物生长。

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