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首页> 外文期刊>Journal of Clinical Microbiology >Enzyme-linked immunoassay for detection of Cryptosporidium antigens in fecal specimens.
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Enzyme-linked immunoassay for detection of Cryptosporidium antigens in fecal specimens.

机译：酶联免疫法检测粪便标本中隐孢子虫抗原。

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Cryptosporidium sp. is a ubiquitous 4- to 6-micron protozoan parasite infecting the intestinal tract of humans. It causes mild to fulminant diarrhea in patients, especially immunocompromised persons, and it may be hard to detect by microscopic fecal examination. An indirect, double-antibody enzyme-linked immunosorbent assay (ELISA) was developed using specifically produced goat and rabbit antisera to detect Cryptosporidium antigens in human feces. Of 62 frozen stools from patients with cryptosporidiosis, as detected by at least two microscopic diagnostic techniques, 51 were positive by ELISA; all ELISA-negative specimens came from patients with fewer than five oocysts per 0.01 ml of concentrated fecal sample examined after modified acid-fast or fluorescent monoclonal antibody staining. A total of 182 specimens from persons without Cryptosporidium infection were negative by ELISA in 176 instances; 3 ELISA-positive specimens came from patients with cryptosporidiosis diagnosed earlier. The sensitivity of the assay was 82.3%, and specificity was 96.7%. The predictive value of a positive ELISA was 89.5%, and the predictive value of a negative ELISA was 94.2%. The ELISA was not affected by the presence of eight other intestinal parasites but was sometimes affected by repeated freezing and thawing of fecal specimens. All fecal specimens were heated to 100 degrees C for 2 min to reduce proteolytic enzyme activity, although the necessity of this step needs further evaluation. This first-generation ELISA is a simple, rapid, easily standardized test for Cryptosporidium antigens in stool samples which will be useful for diagnosis and for large-scale epidemiologic studies.
机译：隐孢子虫是一种普遍存在的4到6微米原生动物寄生虫，可感染人的肠道。它会引起患者的轻度至暴发性腹泻，尤其是免疫功能低下的人，可能很难通过显微镜的粪便检查来检测。使用专门生产的山羊和兔抗血清开发了一种间接的双抗体酶联免疫吸附测定（ELISA），以检测人粪便中的隐孢子虫抗原。通过至少两种显微诊断技术检测到的62例隐孢子虫病冷冻大便中，有51例呈阳性。所有经过ELISA阴性的标本均来自经过改良的抗酸或荧光单克隆抗体染色后每0.01 ml浓缩粪便样本中卵囊少于五个的患者。 176例中，无隐孢子虫感染者的182份标本阴性。 3 ELISA阳性标本来自较早诊断出的隐孢子虫病患者。该测定的灵敏度为82.3％，特异性为96.7％。阳性ELISA的预测值为89.5％，阴性ELISA的预测值为94.2％。 ELISA不受其他八种肠道寄生虫的影响，但有时会受到粪便标本反复冻融的影响。尽管此步骤的必要性需要进一步评估，所有粪便标本均加热到100摄氏度2分钟以降低蛋白水解酶的活性。此第一代ELISA是对粪便样品中隐孢子虫抗原的简单，快速，易于标准化的测试，对于诊断和大规模流行病学研究将非常有用。

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