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首页> 外文期刊>Journal of Clinical Microbiology >Diagnosis of genital Chlamydia trachomatis infections in asymptomatic males by testing urine by PCR.
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Diagnosis of genital Chlamydia trachomatis infections in asymptomatic males by testing urine by PCR.

机译：通过PCR检测尿液可诊断无症状男性生殖道沙眼衣原体感染。

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An enzyme-linked immunosorbent assay (EIA) (MikroTrak; Syva) was compared with PCR (Amplicor; Roche) for detection of Chlamydia trachomatis in first-void urine (FVU) from 184 men attending a skin and venereal disease clinic. The prevalence of C. trachomatis in the population studied was 18.5%. Discrepant results between Syva EIA and Roche PCR were retested by using major outer membrane protein primer-based PCR. After retesting, the sensitivity, the specificity, and the positive and negative predictive values for the Syva EIA were 85.3, 100, 100, and 77.5%, respectively, and those for the Roche PCR 100, 100, 100, and 100%, respectively. It was concluded that PCR provides a highly sensitive and specific noninvasive screening method for genital chlamydial infection in asymptomatic men.
机译：将酶联免疫吸附测定（EIA）（MikroTrak; Syva）与PCR（Amplicor; Roche）进行了比较，以检测184名就诊于皮肤和性病诊所的男性的初次排尿（FVU）中的沙眼衣原体。在研究人群中沙眼衣原体的患病率为18.5％。 Syva EIA和Roche PCR之间的差异结果通过使用主要的外膜蛋白引物为基础的PCR进行了重新测试。重新测试后，Syva EIA的敏感性，特异性以及阳性和阴性预测值分别为85.3、100、100和77.5％，而Roche PCR的灵敏度，特异性和阳性预测值分别为100、100、100和100％ 。结论是，PCR为无症状男性生殖器衣原体感染提供了一种高度敏感和特异性的非侵入性筛查方法。

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