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首页> 外文期刊>Journal of Clinical Microbiology >Accuracy of four commercial systems for identification of Burkholderia cepacia and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis.
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Accuracy of four commercial systems for identification of Burkholderia cepacia and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis.

机译：从囊性纤维化患者中回收的鉴定伯克霍尔德菌洋葱和其他革兰氏阴性非发酵菌的四个商业系统的准确性。

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Burkholderia cepacia has recently been recognized as an important pathogen in chronic lung disease in patients with cystic fibrosis (CF). Because of the social, psychological, and medical implications of the isolation of B. cepacia from CF patients, accurate identification of this organism is essential. We compared the accuracies of four commercial systems developed for the identification of nonfermenting, gram-negative bacilli with that of conventional biochemical testing for 150 nonfermenters including 58 isolates of B. cepacia recovered from respiratory secretions from CF patients. The accuracies of the four systems for identifying all nonfermenters ranged from 57 to 80%, with the RapID NF Plus system being most accurate. The accuracies of these systems for identifying B. cepacia ranged from 43 to 86%, with the Remel system being most accurate. Depending on the commercial system, from two to seven isolates were misidentified as B. cepacia. The relatively poor performance of the commercial systems requires that identification of certain nonfermenters be confirmed by conventional biochemical testing. These organisms include B. cepacia, Burkholderia sp. other than B. cepacia, and infrequently encountered environmental species (Pseudomonas and Flavobacterium species). In addition, conventional biochemical testing should be done if a commercial system fails to assign an identification to an organism. Confirmatory testing should preferably be performed by a reference laboratory with experience in working organisms isolated from CF patients.
机译：洋葱伯克霍尔德菌最近被认为是囊性纤维化（CF）患者慢性肺部疾病的重要病原体。由于从CF患者中分离出洋葱伯克霍尔德菌具有社会，心理和医学意义，因此准确鉴定这种生物至关重要。我们比较了开发的用于鉴定非发酵革兰氏阴性杆菌的四种商业系统与常规生化测试对150种非发酵菌的精确度，其中包括从CF患者呼吸道分泌物中分离出的58株洋葱败血杆菌。识别所有非发酵菌的四个系统的准确度从57％到80％不等，其中RapID NF Plus系统最为准确。这些系统用于鉴定洋葱伯克霍尔德菌的准确性范围为43％至86％，其中Remel系统最为准确。根据商业系统的不同，有2到7个菌株被鉴定为洋葱伯克霍尔德菌。商业系统的相对较差的性能要求通过常规的生化测试确认某些非发酵剂的鉴定。这些生物包括洋葱伯克霍尔德氏菌，伯克霍尔德氏菌。除洋葱伯克霍尔德菌外，还很少遇到环境物种（假单胞菌和黄杆菌属）。此外，如果商业系统无法为生物体分配标识，则应进行常规生化测试。验证性测试最好应由具有从CF患者中分离出的工作有机体经验的参考实验室进行。

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