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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of VITEK 2 Rapid Identification and Susceptibility Testing System against Gram-Negative Clinical Isolates
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Evaluation of VITEK 2 Rapid Identification and Susceptibility Testing System against Gram-Negative Clinical Isolates

机译：针对革兰氏阴性临床分离株的VITEK 2快速鉴定和药敏试验系统的评估

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A total of 281 strains of miscellaneous members of the family Enterobacteriaceae, Pseudomonas aeruginosa, and other gram-negative bacteria were evaluated by use of identification tests with the VITEK 2 system (bioMérieux) and an API identification system (bioMérieux). A total of 237 (95%) strains were correctly identified to the species level. Only six (2.1%) strains were misidentified, and eight (2.8%) strains were not identified. Among 14 strains with discrepant identifications, 8 (57.1%) strains were nonfermenters. The susceptibilities of 228 strains to 11 antibiotics including amikacin, netilmicin, tobramycin, gentamicin, ciprofloxacin, imipenem, meropenem, ceftazidime, cefepime, piperacillin, and piperacillin in combination with tazobactam were tested with the VITEK 2 AST-No. 12 card and by the broth microdilution (MB) method, according to NCCLS guidelines, as a reference. For the 2,508 organism-antibiotic combinations, the rates at which duplicate MICs correlated within ±1 dilution ranged from 84.2 to 95.6%. Only 13 (0.5%) and 10 (0.4%) of the susceptibility tests gave major errors (resistant with the VITEK 2 system but sensitive by the MB method) and very major errors (sensitive with the VITEK 2 system but resistant by the MB method), respectively. Both VITEK 2 ID-GNB (an identification system) and VITEK 2 AST-No. 12 (a susceptibility testing system) card systems gave rapid, reliable, and highly reproducible results.
机译：通过使用VITEK 2系统（bioMérieux ）和API识别系统（bioMérieux）。正确鉴定出了237种（95％）的菌种。只有六种（2.1％）菌株被误认，而八种（2.8％）菌株未被鉴定。在14株鉴定不一致的菌株中，有8株（57.1％）是非发酵菌。使用VITEK 2 AST-No。测试了228株菌株对11种抗生素的敏感性，包括阿米卡星，奈替米星，妥布霉素，庆大霉素，环丙沙星，亚胺培南，美洛培南，头孢他啶，头孢吡肟，哌拉西林和哌拉西林联合他唑巴坦的敏感性。 12卡，并通过肉汤微稀释（MB）方法，根据NCCLS指南，作为参考。对于2,508种生物体-抗生素组合，重复的MIC在±1稀释度内相关的比率为84.2至95.6％。仅13（0.5％）和10（0.4％）的敏感性测试给出了主要错误（对VITEK 2系统有抵抗力，但对MB方法敏感）和非常重要的错误（对VITEK 2系统敏感但对MB法有抵抗力） ）， 分别。 VITEK 2 ID-GNB（识别系统）和VITEK 2 AST-No。 12个（磁敏测试系统）卡片系统给出了快速，可靠和高度可重复的结果。

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