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首页> 外文期刊>Journal of Clinical Microbiology >Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo
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Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo

机译：dubliniensis四种不同基因型的鉴定和体内和体外微进化的检测

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The present study investigates further the population structure of Candida dubliniensis and its ability to exhibit microevolution. Using 98 isolates (including 80 oral isolates) from 94 patients in 15 countries, we confirmed the existence of two distinct populations within the species C. dubliniensis, designated Cd25 group I and Cd25 group II, respectively, on the basis of DNA fingerprints generated with the C. dubliniensis-specific probe Cd25. The majority of Cd25 group I isolates (48 of 71, 67.6%) were from human immunodeficiency virus (HIV)-infected individuals, whereas the majority of Cd25 group II isolates (19 of 27, 70.4%) were from HIV-negative individuals (P ≤ 0.001). Nucleotide sequence analysis of the internal transcribed spacer (ITS) regions of the rRNA genes from 19 representative isolates revealed the presence of four separate genotypes. All of the Cd25 group I isolates tested belonged to genotype 1, while the Cd25 group II population was comprised of three distinct genotypes (genotypes 2 to 4), which corresponded to distinct clades within the Cd25 group II population. These findings were confirmed using genotype-specific PCR primers with 70 isolates. We also showed that C. dubliniensis can exhibit microevolution in vivo and in vitro as occurs in other yeast species. DNA fingerprinting using the C. dubliniensis probes Cd25, Cd24, and Cd1 and karyotype analysis of multiple oral isolates recovered from the same specimen from each of eight separate patients revealed microevolution in six of eight of the clonal populations. Similarly, sequential clonal isolates from various anatomical sites in two separate patients exhibited microevolution. Microevolution was also shown to occur when two clinical isolates susceptible to fluconazole were exposed to the drug in vitro. The epidemiological significance of the four C. dubliniensis genotypes and the ability of C. dubliniensis to undergo microevolution has yet to be established.
机译：本研究进一步研究了 Candida dubliniensis 的种群结构及其表现出微进化的能力。我们使用了来自15个国家/地区的94位患者的98种分离株（包括80种口腔分离株），我们证实了 C物种内存在两个不同的种群。 dubliniensis ，根据 C产生的DNA指纹分别命名为Cd25 I组和Cd25 II组。 dubliniensis 特异性探针Cd25。大部分Cd25 I类分离株（71个中的48个，占67.6％）来自感染人类免疫缺陷病毒（HIV）的个体，而大多数Cd25 II类分离株（27个中的19个，占70.4％）来自HIV阴性个体（ P ≤0.001）。来自19个代表性分离株的rRNA基因的内部转录间隔区（ITS）区域的核苷酸序列分析显示，存在四种不同的基因型。测试的所有Cd25 I类分离物均属于基因型1，而Cd25 II类人群由三种不同的基因型（基因型2至4）组成，分别对应于Cd25 II类人群中的不同进化枝。使用具有70个分离株的基因型特异性PCR引物证实了这些发现。我们还显示了 C。 dubliniensis 与其他酵母菌一样，在体内和体外均表现出微进化。使用 C进行DNA指纹分析。 dubliniensis 探针Cd25，Cd24和Cd1的核型分析以及从八名单独患者中每名患者的同一标本中回收的多个口腔分离株的核型分析显示，在八名克隆人群中有六名发生了微进化。同样，在两名不同患者中，来自各个解剖部位的顺序克隆分离株表现出微进化。当两个对氟康唑敏感的临床分离株在体外暴露于药物时，也发生了微进化。四个 C的流行病学意义。 dubliniensis 基因型和 C的能力。 dubliniensis 尚待确定。

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