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首页> 外文期刊>Journal of Clinical Microbiology >Extended Virulence Genotype of Pathogenic Escherichia coli Isolates Carrying the afa-8 Operon: Evidence of Similarities between Isolates from Humans and Animals with Extraintestinal Infections
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Extended Virulence Genotype of Pathogenic Escherichia coli Isolates Carrying the afa-8 Operon: Evidence of Similarities between Isolates from Humans and Animals with Extraintestinal Infections

机译：携带afa-8操纵子的致病性大肠杆菌分离株的扩大毒力基因型：人和动物肠外感染分离株之间相似性的证据

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The afimbrial AfaE-VIII adhesin is common among Escherichia coli isolates from calves with intestinal and/or extraintestinal infections and from humans with sepsis or pyelonephritis. The virulence genotypes of 77 Escherichia coli afa-8 isolates from farm animals and humans were compared to determine whether any trait of commonality exists between isolates of the different host species. Over half of the extraintestinal afa-8 isolates were associated with pap and f17Ac adhesin genes and contained virulence genes (pap, hly, and cnf1) which are characteristic of human extraintestinal pathogenic E. coli (ExPEC). PapG, which occurs as three known variants (variants I to III), is encoded by the corresponding three alleles of papG. Among the pap-positive strains, new papG variants (papGrs) that differed from the isolates with genes for the three adhesin classes predominated over isolates with papG allele III, which in turn were more prevalent than those with allele II. The data showed the substantial prevalence of the enteroaggregative E. coli heat-stable enterotoxin gene (east1) among afa-8 isolates. Most of the afa-8 isolates harbored the high-pathogenicity island (HPI) present in pathogenic Yersinia; however, two-thirds of the HPI-positive strains shared a truncated HPI integrase gene. The presence of ExPEC-associated virulence factors (VFs) in extraintestinal isolates that carry genes typical of enteric strains and that express O antigens associated with intestinal E. coli is consistent with transfer of VFs and O-antigen determinants between ExPEC and enteric strains. The similarities between animal and human ExPEC strains support the hypothesis of overlapping populations, with members of certain clones or clonal groups including animal and human strains. The presence of multiple-antibiotic-resistant bovine afa-8 strains among such clones may represent a potential public health risk.
机译：房颤AfaE-VIII粘附素在带有肠和/或肠外感染的犊牛以及患有败血症或肾盂肾炎的人的大肠杆菌中很常见。比较了来自农场动物和人类的77种大肠杆菌afa-8 菌株的毒力基因型，以确定不同宿主物种的菌株之间是否存在任何公共性状。肠外 afa-8 分离株的一半以上与 pap 和 f17Ac 黏附素基因相关，并含有毒力基因（ pap ， hly 和 cnf1 ），它们是人肠外致病性 E的特征。大肠杆菌（ExPEC）。 PapG是三个已知变体（变体I至III），由 papG 的三个等位基因编码。在 pap 阳性菌株中，新的 papG 变体（ papGrs ）与具有三种粘附素类基因的分离株不同，而在 papG 等位基因III，后者比等位基因II更为普遍。数据表明，肠聚集性 E的患病率很高。 afa-8 分离株中的大肠杆菌热稳定肠毒素基因（ east1 ）。大多数 afa-8 分离株都带有致病性耶尔森氏菌中存在的高致病性岛（HPI）。但是，三分之二的HPI阳性菌株共享一个截短的HPI整合酶基因。肠外分离物中存在与ExPEC相关的毒力因子（VF），这些因子携带肠病毒株的典型基因并表达与肠道E相关的O抗原。大肠埃希菌与ExPEC和肠溶菌之间VF和O-抗原决定簇的转移是一致的。动物和人类ExPEC菌株之间的相似性支持了种群重叠的假说，其中某些克隆或克隆群的成员包括动物和人类菌株。这些克隆中多重抗药性牛 afa-8 菌株的存在可能代表潜在的公共健康风险。

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