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首页> 外文期刊>Journal of Clinical Microbiology >Culture-Independent Molecular Analysis of Microbial Constituents of the Healthy Human Outer Ear
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Culture-Independent Molecular Analysis of Microbial Constituents of the Healthy Human Outer Ear

机译：健康人外耳微生物成分的文化独立分子分析

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摘要

Molecular-phylogenetic sequence analyses have provided a new perspective on microbial communities by allowing the detection and identification of constituent microorganisms in the absence of cultivation. In this study we used broad-specificity amplification of ribosomal DNA (rDNA) genes to survey organisms present in the human outer ear canal. Samples were obtained from 24 individuals, including members of three extended families, in order to survey the resident microbiota and to examine microbial population structures in individuals related by familial or household associations. To examine the stability of the microbial populations, one individual was sampled four times and another twice over a 14-month period. We found that a distinct set of microbial types was present in the majority of the subjects sampled. The two most prevalent rDNA sequence types that were identified in multiple individuals corresponded closely to those of Alloiococcus otitis and Corynebacterium otitidis, commonly thought to be associated exclusively with infections of the middle ear. Our results suggest, therefore, that the outer ear canal may serve as a reservoir for normally commensal microbes that can contribute to pathogenesis upon introduction into the middle ear. Alternatively, culture analyses of diseases of the middle ear may have been confounded by these contaminating commensal organisms.
机译：分子系统发生序列分析通过允许在没有培养的情况下检测和鉴定组成微生物，为微生物群落提供了新的视角。在这项研究中，我们使用了核糖体DNA（rDNA）基因的广泛特异性扩增来调查人外耳道中存在的生物。从24个个体（包括三个大家庭的成员）中获取样本，以便调查居民微生物群落并检查与家庭或家庭协会相关的个体中的微生物种群结构。为了检查微生物种群的稳定性，在14个月的时间内对一个人进行了四次采样，对另一人进行了两次采样。我们发现在大多数采样对象中存在一组独特的微生物类型。在多个个体中鉴定出的两种最流行的rDNA序列类型与 Allococcus otitis 和 Corynebacterium otitidis 密切相关，通常被认为仅与中耳感染相关。因此，我们的研究结果表明，外耳道可作为正常共生微生物的储存库，这些微生物在引入中耳后可促进发病。或者，这些污染的共生生物可能混淆了对中耳疾病的文化分析。

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