Rapid Discrimination of Monkey B Virus from Human Herpes Simplex Viruses by PCR in the Presence of Betaine

Makoto Hirano; Shin Nakamura; Maki Okada; Masahiro Ueda; Ryozaburo Mukai

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Rapid Discrimination of Monkey B Virus from Human Herpes Simplex Viruses by PCR in the Presence of Betaine

机译：在甜菜碱存在下通过PCR快速鉴别人单纯疱疹病毒中的猴B病毒

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A PCR method to amplify DNA segments of the glycoprotein G gene of monkey B virus (BV) was achieved by adding betaine to the PCR mixture, in spite of the high G+C content of this gene. No product was obtained when DNA of human herpes simplex viruses (HSVs) was used as the template under the same conditions. Thus, this PCR method is useful in discriminating BV from HSVs.

机译：尽管该基因的G + C含量很高，但仍通过向PCR混合物中添加甜菜碱来实现一种扩增猴B病毒（BV）糖蛋白G基因的DNA片段的PCR方法。当在相同条件下使用人单纯疱疹病毒（HSV）的DNA作为模板时，未获得任何产物。因此，该PCR方法可用于区分BV和HSV。

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《Journal of Clinical Microbiology》 |2000年第3期|共3页
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Makoto Hirano; Shin Nakamura; Maki Okada; Masahiro Ueda; Ryozaburo Mukai;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Electrochemical DNA biosensor for the detection and discrimination of herpes simplex Type I and Type II viruses from PCR amplified real samples [J] . Pinar Kara, Burcu Meric, Aysin Zeytinoglu, Analytica chimica acta . 2004,第1a2期

机译：电化学DNA生物传感器，用于从PCR扩增的真实样品中检测和区分单纯型I型和II型疱疹病毒
2. Imaging of the varicella zoster virion in the viral highways: comparison with herpes simplex viruses 1 and 2, cytomegalovirus, pseudorabies virus, and human herpes viruses 6 and 7. [J] . Padilla JA, Nii S, Grose C Journal of Medical Virology . 2003,第Suppla1期

机译：病毒高速公路中水痘带状疱疹病毒粒子的成像：与单纯疱疹病毒1和2，巨细胞病毒，假狂犬病毒以及人疱疹病毒6和7的比较。
3. Rapid quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6 DNA in blood and other clinical specimens. [J] . Engelmann I, Petzold DR, Kosinska A, Journal of Medical Virology . 2008,第3期

机译：快速定量PCR分析法可同时检测血液和其他临床标本中的单纯疱疹病毒，水痘带状疱疹病毒，巨细胞病毒，爱泼斯坦-巴尔病毒和人疱疹病毒6 DNA。
4. Ability of Organotin Pyrimidine Polyamines to Inhibit HSV-1 (Herpes Simplex Virus) and Vaccinia (Small Pox Virus) Viruses [C] . Kimberly Shahi, Michael R. Roner, Amitabh Battin PMSE Symposia . 2008

机译：有机锡嘧啶多胺能够抑制HSV-1（单纯疱疹病毒）和痘苗病毒（小痘病毒）病毒的能力
5. Antiviral activities of selected Hong Kong marine algae against herpes simplex viruses and other viruses and their possible antiviral mechanisms. [D] . Zhu, Wen. 2002

机译：某些香港海藻对单纯疱疹病毒和其他病毒的抗病毒活性及其可能的抗病毒机制。
6. Note: Rapid Discrimination of Monkey B Virus from Human Herpes Simplex Viruses by PCR in the Presence of Betaine [O] . Makoto Hirano, Shin Nakamura, Maki Okada, 2000

机译：注意：在甜菜碱存在下通过PCR快速区分人单纯疱疹病毒中的猴B病毒
7. A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses [O] . Anshu Gupta, Shelley M. Lawrence, Stephanie I. Fraley 2020

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8. Report on Carcinogens (RoC) Concept: Selected Viruses. Epstein--Barr Virus, Human ImmunodeficiencynVirus Type 1, Human T--Cell Lymphotropic Virus Type 1, Kaposi Sarcoma--Associated Herpes Virus, and nMerkel Cell Polyoma Virus. [R] . 2016

机译：关于致癌物（RoC）概念的报告：选定的病毒。爱泼斯坦 - 巴尔病毒，人类免疫缺陷病毒1型，人类T细胞淋巴细胞病毒1型，卡波西肉瘤 - 相关疱疹病毒和nmerkel细胞多瘤病毒。

Rapid Discrimination of Monkey B Virus from Human Herpes Simplex Viruses by PCR in the Presence of Betaine

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