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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Precytopathic Effect of Enteroviruses in Clinical Specimens by Centrifugation-Enhanced Antigen Detection
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Detection of Precytopathic Effect of Enteroviruses in Clinical Specimens by Centrifugation-Enhanced Antigen Detection

机译：离心增强抗原检测法检测临床样本中肠病毒的前细胞病变作用

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Rapid enterovirus detection is important for decisions about antibiotic administration and length of hospital stay. The efficacy of rapid antigen detection-cell culture amplification (Ag-CCA) was evaluated with monoclonal antibodies (MAbs) 5-D8/1 (DAKO) and Pan-Enterovirus clone 2E11 (Chemicon) with 10 poliovirus, echovirus, and coxsackievirus type A and B stock isolates and College of American Pathologists check samples. By using Ag-CCA technology, MAb 2E11 was more sensitive than 5-D8/1 at detecting a greater number of stock isolates at or past tube (cytopathic effect [CPE]) culture (TC) end points. The efficacy of Ag-CCA in the clinical setting was subsequently confirmed with 273 consecutively freshly collected nasopharyngeal aspirate or swab specimens, rectal swab, and cerebrospinal fluid specimens during the 1999 enterovirus season. All specimens were tested by Ag-CCA in parallel with rhesus monkey kidney (RhMk), MRC-5, and A549 conventional TCs. Approximately 60% of field specimens were additionally tested with Hep-2 and HNK conventional TCs. Sixty-two percent of the clinical specimens tested were Ag-CCA positive after 48 h. Among 51 isolates, the mean time to CPE or culture confirmation was 5.5 days (range, 2 to 18 days). After 48 h, Ag-CCA achieved sensitivity, specificity, and positive and negative predictive values of 62, 100, 100, and 93%, respectively. During the same period, TC-CPE displayed test parameters of 12, 100, 100, and 85%, respectively. After 5 days, the sensitivity and specificity of Ag-CCA increased to 92 and 98%, respectively. Within the same period, isolation attained sensitivity and specificity of 52 and 100%, respectively. Although Ag-CCA displayed slightly reduced sensitivity and reduced specificity compared with conventional cell culture after 14 days, the markedly superior 48-h enterovirus Ag-CCA detection rate supports incorporation of this assay into the routine clinical setting.
机译：快速的肠道病毒检测对于决定抗生素使用和住院时间很重要。使用单克隆抗体（MAb）5-D8 / 1（DAKO）和带有10种脊髓灰质炎病毒，回声病毒和柯萨奇病毒A型的泛肠病毒克隆2E11（Chemicon）评估了快速抗原检测细胞培养扩增（Ag-CCA）的功效和B株分离株，美国病理学家学院检查样本。通过使用Ag-CCA技术，MAb 2E11比5-D8 / 1在检测更多或更多的管分离物（细胞病变效应[CPE]）培养（TC）终点时更敏感。随后在1999年肠道病毒季节期间，连续273份新鲜收集的鼻咽抽吸物或拭子标本，直肠拭子和脑脊液标本证实了Ag-CCA在临床上的疗效。所有标本均通过Ag-CCA与恒河猴肾（RhMk），MRC-5和A549常规TC平行测试。约有60％的野外标本还使用Hep-2和HNK常规TC进行了测试。 48小时后，有62％的临床标本为Ag-CCA阳性。在51个分离株中，平均CPE或培养确认时间为5.5天（范围为2到18天）。 48小时后，Ag-CCA的敏感性，特异性以及阳性和阴性预测值分别达到62％，100％，100％和93％。在同一时期，TC-CPE显示的测试参数分别为12、100、100和85％。 5天后，Ag-CCA的敏感性和特异性分别提高到92％和98％。在同一时期内，分离获得的敏感性和特异性分别为52％和100％。尽管与14天后的常规细胞培养相比，Ag-CCA的敏感性和特异性稍有降低，但48小时肠病毒Ag-CCA的检测率明显更高，支持将这种检测方法纳入常规临床环境。

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