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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Automated and Nonautomated Systems for Identification of Burkholderia pseudomallei
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Comparison of Automated and Nonautomated Systems for Identification of Burkholderia pseudomallei

机译：自动化和非自动化系统鉴定假伯克霍尔德氏菌的比较

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摘要

The identification of Burkholderia pseudomallei, the causative agent of melioidosis, is usually not difficult in laboratories in areas where it is endemic. With the increase in international travel and the threat of bioterrorism, it has become more likely that laboratories in areas where it is not endemic could encounter this organism. The increase in the use of and dependence upon automated identification systems makes accurate identification of uncommonly encountered organisms such as B. pseudomallei critically important. This study compares the manual API 20NE and 20E identification systems with the automated Vitek 1 and 2 systems. A total of 103 B. pseudomallei isolates were tested and correctly identified in 98%, 99%, 99%, and 19% of cases, respectively. The failure of the Vitek 2 to correctly identify B. pseudomallei was largely due to differences in the biochemical reactions achieved compared to expected values in the database. It is suggested that this deficiency in the Vitek 2 may be due to the large number of uncertain results reported for these isolates. These results reduce the discriminating ability of the instrument to distinguish between uncommonly encountered isolates such as those of B. pseudomallei.
机译：识别类鼻疽病的病原体 Burkholderia pseudomallei 通常在地方病流行的实验室中并不难。随着国际旅行的增加和生物恐怖主义的威胁，非地方性地区的实验室更有可能遇到这种生物。自动识别系统的使用和依赖性的增加使得能够准确识别不常见的生物，例如 B。假苹果酱至关重要。这项研究将手动API 20NE和20E识别系统与自动化Vitek 1和2系统进行了比较。总共103个 B。分别在98％，99％，99％和19％的病例中检测并正确鉴定了假苹果芽孢杆菌。 Vitek 2无法正确识别 B。假马来酸主要是由于与数据库中的预期值相比，所实现的生化反应存在差异。建议Vitek 2中的这种缺陷可能是由于报告了这些分离株的大量不确定结果。这些结果降低了仪器区分罕见的分离株（例如 B分离株）的能力。假苹果花。

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