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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Epidemiology of Pneumococcal Carriage among Children with Upper Respiratory Tract Infections in Hanoi, Vietnam
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Molecular Epidemiology of Pneumococcal Carriage among Children with Upper Respiratory Tract Infections in Hanoi, Vietnam

机译：越南河内患有上呼吸道感染的儿童肺炎球菌携带的分子流行病学

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To investigate the molecular epidemiology of pneumococcal nasopharyngeal carriage in Hanoi, Vietnam, we studied 84 pneumococcal strains retrieved from children with upper respiratory tract infections. Serotypes 23F (32%), 19F (21%), 6B (13%), and 14 (10%) were found most often. A significant number of strains were antibiotic resistant. Fifty-two percent of the strains were (intermediate) resistant to penicillin, 87% were (intermediate) resistant to co-trimoxazole, 76% were resistant to tetracycline, 73% were resistant to erythromycin, and 39% were (intermediate) resistant to cefotaxime. Seventy-five percent were resistant to three or more classes of antibiotics. A high degree of genetic heterogeneity among the penicillin resistance genes was observed. In addition, the tetracycline resistance gene tet(M) and the erythromycin resistance gene erm(B) were predominantly observed among the isolates. Molecular analysis of the 84 isolates by restriction fragment end labeling (RFEL) revealed 35 distinct genotypes. Twelve of these genotypes represented a total of eight genetic clusters with 61 isolates (73%). The two largest clusters contained 24 and 12 isolates, and the isolates in those clusters were identical to the two internationally spreading multidrug-resistant clones Spain 23F-1 and Taiwan 19F-14, respectively. The remaining RFEL types were Vietnam specific, as they did not match the types in our reference collection of 193 distinct RFEL types from 16 countries. Furthermore, 57 of the 61 horizontally spreading isolates (93%) in the eight genetic clusters were covered by the seven-valent conjugate vaccine, whereas this vaccine covered only 43% of the isolates with unique genotypes. According to the serotype distribution of the nasopharyngeal pneumococcal isolates, this study suggests a high potential benefit of the seven-valent pneumococcal conjugate vaccine for children in Hanoi.
机译：为了调查越南河内肺炎球菌鼻咽运输的分子流行病学，我们研究了从患有上呼吸道感染的儿童中回收的84株肺炎球菌菌株。最常见的血清型为23F（32％），19F（21％），6B（13％）和14（10％）。大量菌株具有抗生素抗性。 52％的菌株对青霉素耐药（中级），87％的对三甲唑唑耐药（中级），76％的对四环素耐药，73％的对红霉素耐药，39％的（中）耐药头孢噻肟。 75％的人对三种或三种以上的抗生素有抗药性。观察到青霉素抗性基因之间的高度遗传异质性。此外，在分离物中主要观察到四环素抗性基因 tet （M）和红霉素抗性基因 erm （B）。通过限制性片段末端标记（RFEL）对84个分离株的分子分析揭示了35种不同的基因型。这些基因型中的十二个代表总共八个遗传簇，其中有61个分离株（73％）。两个最大的簇包含24个和12个分离株，这些簇中的分离株分别与两个国际传播的耐多药克隆西班牙23F-1和台湾19F-14相同。其余的RFEL类型是越南特定的，因为它们与我们参考的来自16个国家的193种不同RFEL类型的参考馆藏中的类型不匹配。此外，七价结合疫苗覆盖了八个基因簇中水平分布的61个分离株中的57个（93％），而该疫苗仅覆盖了43％具有独特基因型的分离株。根据鼻咽肺炎球菌分离株的血清型分布，这项研究表明七价肺炎球菌结合疫苗对河内儿童具有很高的潜在益处。

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