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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Epstein-Barr Virus Genomes in Peripheral Blood B Cells from Solid-Organ Transplant Recipients by Fluorescence In Situ Hybridization
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Detection of Epstein-Barr Virus Genomes in Peripheral Blood B Cells from Solid-Organ Transplant Recipients by Fluorescence In Situ Hybridization

机译：荧光原位杂交技术检测固体器官移植受者外周血B细胞中爱泼斯坦-巴尔病毒基因组

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Resolution of Epstein-Barr Virus (EBV) infection in pediatric solid-organ transplant recipients often leads to an asymptomatic carrier state characterized by a persistently elevated circulating EBV load that is 2 to 4 orders of magnitude greater than the load typical of healthy latently infected individuals. Elevated EBV loads in immunosuppressed individuals are associated with an increased risk for development of posttransplant lymphoproliferative disease. We have performed fluorescence in situ hybridization (FISH) studies with peripheral blood B cells from carriers of persistent EBV loads in order to directly quantitate the number of EBV genomes per infected cell. Patients were assigned to two groups on the basis of the level of the persistent load (low-load carriers, 8 to 200 genomes/105 peripheral blood lymphocytes; high-load carriers, >200 genomes/105 peripheral blood lymphocytes). FISH analysis revealed that the low-load carriers predominantly had circulating virus-infected cells harboring one or two genome copies/cell. High-load carriers also had cells harboring one or two genome copies/cell; in addition, however, they carried a distinct population of cells with high numbers of viral genome copies. The increased viral loads correlated with an increase in the frequency of cells containing high numbers of viral genomes. We conclude that low-load carriers possess EBV-infected cells that are in a state similar to normal latency, whereas high-load carriers possess two populations of virus-positive B cells, one of which carries an increased number of viral genomes per cell and is not typical of normal latency.
机译：小儿实体器官移植受者的爱泼斯坦-巴尔病毒（EBV）感染消退常导致无症状携带者状态，其特征是循环EBV持续升高，比健康潜伏感染个体的典型载荷高2至4个数量级。 。免疫抑制个体的EBV负荷升高与移植后淋巴细胞增生性疾病发展的风险增加相关。为了直接定量每个感染细胞的EBV基因组数量，我们已经对来自持久性EBV负荷携带者的外周血B细胞进行了荧光原位杂交（FISH）研究。根据持续负荷水平将患者分为两组（低负荷携带者，8至200个基因组/ 10 5 外周血淋巴细胞;高负荷携带者，> 200个基因组/ 10 5 外周血淋巴细胞）。 FISH分析表明，低负荷携带者主要具有循环病毒感染的细胞，每个细胞带有一个或两个基因组拷贝。高负荷携带者还具有每个细胞带有一个或两个基因组拷贝的细胞。然而，此外，它们携带了具有大量病毒基因组拷贝的独特细胞群。病毒载量的增加与包含大量病毒基因组的细胞频率的增加有关。我们得出的结论是，低负荷携带者拥有EBV感染的细胞，其状态类似于正常潜伏期，而高负荷携带者拥有两群病毒阳性的B细胞，其中每一个携带的病毒基因组数量增加，并且不是正常延迟的典型表现。

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