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首页> 外文期刊>Journal of Clinical Microbiology >Use of Turbidimetric Growth Curves for Early Determination of Antifungal Drug Resistance of Filamentous Fungi
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Use of Turbidimetric Growth Curves for Early Determination of Antifungal Drug Resistance of Filamentous Fungi

机译：用比浊法生长曲线早期测定丝状真菌的抗药性

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A previously described microbroth kinetic system (J. Meletiadis, J. F. Meis, J. W. Mouton, and P. E. Verweij, J. Clin. Microbiol. >39:478-484, 2001) based on continuous monitoring of changes in the optical density of fungal growth was used to describe turbidimetric growth curves of different filamentous fungi in the presence of increasing concentrations of antifungal drugs. Therefore, 24 clinical mold isolates, including Rhizopus oryzae, Aspergillus fumigatus, Aspergillus flavus, and Scedosporium prolificans, were tested against itraconazole, terbinafine, and amphotericin B according to NCCLS guidelines. Among various parameters of the growth curves, the duration of the lag phase was strongly affected by the presence of antifungal drugs. Exposure to increasing drug concentrations resulted in prolonged lag phases of the turbidimetric growth curves. The lag phases of the growth curves at drug concentrations which resulted in more than 50% growth (for itraconazole and terbinafine) and more than 75% growth (for amphotericin B) after 24 h of incubation for R. oryzae, 48 h for Aspergillus spp., and 72 h for S. prolificans were 4 h longer than the lag phases of the growth curves at the corresponding drug-free growth controls which varied from 4.4 h for R. oryzae, 6.5 h for A. flavus, 7.9 h for A. fumigatus, and 11.6 h for S. prolificans. The duration of the lag phases showed small experimental and interstrain variability, with differences of less than 2 h in most of the cases. Using this system, itraconazole and terbinafine resistance (presence of >50% growth) as well as amphotericin B resistance (presence of >75% growth) was determined within incubation periods of 5.0 to 7.7 h for R. oryzae (for amphotericin B resistance incubation for up to 12 h was required), 8.8 to 11.4 h for A. fumigatus, 6.7 to 8.5 h for A. flavus, and 13 to 15.6 h for S. prolificans while awaiting formal MIC determination by the NCCLS reference method.
机译：基于连续监测的变化的先前描述的微泡动力学系统（J.Meletiadis，JF Meis，JW Mouton和PE Verweij，J.Clin.Microbiol。> 39： 478-484，2001）。真菌生长的光密度用于描述在增加浓度的抗真菌药物存在下不同丝状真菌的浊度生长曲线。因此，有24种临床霉菌分离株，包括米根霉，烟曲霉，黄曲霉和产酸孢子菌。根据NCCLS指南对伊曲康唑，特比萘芬和两性霉素B进行了测试。在生长曲线的各种参数中，迟滞期的持续时间受到抗真菌药物的存在的强烈影响。暴露于增加的药物浓度会导致浊度生长曲线的滞后阶段延长。在 R孵育24小时后，药物浓度下的生长曲线的滞后阶段导致50％以上的增长（伊曲康唑和特比萘芬）和75％以上的增长（两性霉素B）。米曲霉，曲霉菌48小时，曲霉菌72小时。相应的无毒生长对照中，多能生比生长曲线的滞后期长4 h，而 R的生长期则为4.4 h。米， A 6.5小时。黄蜂， A 7.9小时。烟熏， S为11.6小时。多产的。滞后阶段的持续时间显示出较小的实验和菌株间变异性，在大多数情况下差异小于2 h。使用该系统，在 R的孵育时间5.0至7.7小时内，确定了伊曲康唑和特比萘芬的耐药性（> 50％的增长）以及两性霉素B的耐药性（> 75％的增长）。米糊（对于两性霉素B的抗性孵育需要长达12小时）， A则需要8.8-11.4 h。烟： A为6.7至8.5小时。黄褐变， S持续13到15.6 h。在等待NCCLS参考方法正式确定MIC的过程中。

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