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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of the MicroScan Rapid Neg ID3 Panel for Identification of Enterobacteriaceae and Some Common Gram-Negative Nonfermenters
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Evaluation of the MicroScan Rapid Neg ID3 Panel for Identification of Enterobacteriaceae and Some Common Gram-Negative Nonfermenters

机译：用于鉴定肠杆菌科细菌和一些常见的革兰氏阴性非发酵菌的MicroScan快速Neg ID3面板的评估

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The MicroScan Rapid Neg ID3 panel (Dade Behring, Inc., West Sacramento, Calif.) is designed for the identification of gram-negative bacilli. We evaluated its ability to accurately identifyEnterobacteriaceae that are routinely encountered in a clinical laboratory and glucose nonfermenting gram-negative bacilli. Using 511 stock cultures that were maintained at ?70°C and passaged three times before use, we inoculated panels according to the manufacturer's instructions and processed them in a Walk/Away instrument using version 22.01 software. The time to identification was 2 h and 30 min. All panel identifications were compared to reference identifications previously determined by conventional tube biochemicals. At the end of the initial 2.5-h incubation period, 405 (79.3%) identifications were correct. An additional 49 (9.6%) isolates were correctly identified after required additional off-line biochemical tests were performed. Thus, at 24 h, 88.8% of the 511 strains tested were correctly identified. Twenty-two (4.3%) were identified to the genus level only. Twenty-six (5.1%) strains were misidentified. Because the system is based on fluorogenics, there are no conventional tests readily available with which to compare possibly incorrect reactions. Of the 28 Salmonella strains that were tested, 5 were incorrectly reported. The 21 remaining errors were scattered among the genera tested. Testing on nine strains gave a result of “no identification” (very rare biotype). The Rapid Neg ID3 panel in this study approached 89% accuracy for the identification of gram-negative organisms encountered in the hospital laboratory.
机译：MicroScan Rapid Neg ID3面板（加利福尼亚州西萨克拉曼多的Dade Behring，Inc.）设计用于鉴定革兰氏阴性杆菌。我们评估了其准确鉴定在临床实验室中经常遇到的肠杆菌科和葡萄糖非发酵革兰氏阴性杆菌的能力。使用511种保持在70°C的培养物，并在使用前先传代3次，我们按照制造商的说明接种板，并使用22.01版软件在Walk / Away仪器中对其进行处理。识别时间为2小时30分钟。将所有小组鉴定结果与先前由常规试管生化试剂确定的参考鉴定结果进行比较。在最初的2.5小时潜伏期结束时，有405例（79.3％）的鉴定是正确的。在进行了额外的离线生化测试之后，正确识别出了另外49个（9.6％）分离株。因此，在24 h时，正确鉴定了511个被测菌株中的88.8％。仅鉴定到属水平的有22个（4.3％）。 26株（5.1％）菌株被误认。因为该系统基于荧光物质，所以没有现成的常规测试可用来比较可能不正确的反应。在测试的28株沙门氏菌菌株中，有5例报告错误。其余21个错误分散在测试的属中。对九种菌株的测试结果为“未鉴定”（非常罕见的生物型）。在这项研究中，Rapid Neg ID3小组用于鉴定在医院实验室中遇到的革兰氏阴性菌的准确性接近89％。

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