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首页> 外文期刊>Journal of Clinical Microbiology >Isolation and Molecular Characterization ofClostridium difficile Strains from Patients and the Hospital Environment in Belarus
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Isolation and Molecular Characterization ofClostridium difficile Strains from Patients and the Hospital Environment in Belarus

机译：白俄罗斯艰难梭菌菌株的分离，分子鉴定及医院环境

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Toxigenic Clostridium difficile is the most common etiologic agent of hospital-acquired diarrhea in developed countries. The role of this pathogen in nosocomial diarrhea in Eastern Europe has not been clearly established. The goal of this study was to determine the prevalence of C. difficile in patients and the hospital environment in Belarus and to characterize these isolates as to the presence of toxin genes and their molecular type. C. difficile was isolated from 9 of 509 (1.8%) patients analyzed and recovered from 28 of 1,300 (2.1%) environmental sites cultured. A multiplex PCR assay was used to analyze the pathogenicity locus (PaLoc) of all isolates, and strain identity was determined by an arbitrarily primed PCR (AP-PCR). The targeted sequences for all the genes in the PaLoc were amplified in all C. difficile strains examined. A predominantly homogenous group of strains was found among these isolates, with five major AP-PCR groups being identified. Eighty-three percent of environmental isolates were classified into two groups, while patient isolates grouped into three AP-PCR types, two of which were also found in the hospital environment. Although no data on the role of C. difficile infection or epidemiology of C. difficile-associated diarrhea (CDAD) in this country exist, the isolation of toxigenic C. difficile from the hospital environment suggests that this pathogen may be responsible for cases of diarrhea of undiagnosed origin and validates our effort to further investigate the significance of CDAD in Eastern Europe.
机译：产毒素的艰难梭菌是发达国家医院获得性腹泻最常见的病因。在东欧，这种病原体在医院腹泻中的作用尚未明确。这项研究的目的是确定 C的患病率。在白俄罗斯的患者和医院环境中存在困难，并根据毒素基因的存在及其分子类型对这些分离株进行表征。 C。从分析的509名患者中的9名（1.8％）中分离出艰难梭菌，并从1300个（2.1％）培养的环境场所中的28个中恢复。使用多重PCR分析法分析所有分离株的致病性基因座（PaLoc），并通过任意引物PCR（AP-PCR）确定菌株同一性。 PaLoc中所有基因的靶向序列均在所有C中扩增。检查了艰难梭菌菌株。在这些分离株中发现了主要同源的菌株组，其中鉴定出五个主要的AP-PCR组。 83％的环境分离株被分为两组，而患者分离株被分为三种AP-PCR类型，其中两种在医院环境中也被发现。尽管没有关于 C作用的数据。艰难梭菌感染或 C的流行病学。该国存在艰难梭菌相关性腹泻（CDAD），分离出有毒的 C。医院环境的困难提示该病原体可能是导致原发性腹泻的原因，并证实了我们为进一步研究CDAD在东欧的意义所做的努力。

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