Application of Representational Difference Analysis to Genomic Fragments of Marek's Disease Virus

Daiji Endoh; Kyoung-Oh Cho; Kenji Tsukamoto; Toshifumi Morimura; Yasuhiro Kon; Masanobu Hayashi

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Application of Representational Difference Analysis to Genomic Fragments of Marek's Disease Virus

机译：代表性差异分析在马立克氏病病毒基因组片段中的应用

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A rapid and simple method for isolation of DNA fragments of Marek's disease virus (MDV) based on representational difference analysis (RDA) was developed. Multiple viral DNA fragments, the sizes of which were restricted to 0.3 to 3.5 kbp, were simultaneously amplified after subtraction of chicken DNA from BamHI-,BglII-, EcoRI-, HindIII-, orXhoI-digested DNA fragments of MDV-infected cells. Nucleotide sequence of two RDA-derived fragments coincided with the sequence determined from direct sequencing of the MDV genome. We detected an interstrain difference in the size of restriction enzyme-digested fragments on agarose gel. This method was used on a single feather pulp to generate sufficient MDV DNA for cloning.

机译：建立了一种基于代表性差异分析（RDA）的马立克氏病病毒（MDV）DNA片段的快速简便分离方法。从 Bam HI-， Bgl II-，减去鸡DNA后，同时扩增了多个病毒DNA片段，其大小限制在0.3至3.5 kbp。 > Eco RI-， Hin dIII-或 Xho I消化的MDV感染细胞的DNA片段。两个RDA衍生片段的核苷酸序列与从MDV基因组直接测序确定的序列一致。我们在琼脂糖凝胶上检测到了限制性内切酶消化片段大小的菌株间差异。此方法用于单个羽毛纸浆，以生成足够的MDV DNA进行克隆。 展开▼

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《Journal of Clinical Microbiology》 |2000年第12期|共5页

作者
Daiji Endoh; Kyoung-Oh Cho; Kenji Tsukamoto; Toshifumi Morimura; Yasuhiro Kon; Masanobu Hayashi;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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2. Application of representational difference analysis to identify genomic differences between Bradyrhizobium elkanii and B. Japonicum species [J] . Soares René Arderius, Passaglia Luciane Maria Pereira Revista de microbiologia . 2010,第4期

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3. Establishment of methylation-sensitive-representational difference analysis and isolation of hypo- and hypermethylated genomic fragments in mouse liver tumors [J] . Toshikazu Ushijima, Keiichirou Morimura, Yoko Hosoya Proceedings of the National Academy of Sciences of the United States of America . 1997,第6期

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6. Application of Representational Difference Analysis to Genomic Fragments of Mareks Disease Virus [O] . Daiji Endoh, Kyoung-Oh Cho, Kenji Tsukamoto, 2000

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7. Application of representational difference analysis to identify genomic differences between Bradyrhizobium elkanii and B. Japonicum species [O] . René Arderius Soares, Luciane Maria Pereira Passaglia 2010

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Application of Representational Difference Analysis to Genomic Fragments of Marek's Disease Virus

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