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首页> 外文期刊>Journal of Clinical Microbiology >Utilization of Exocellular Mannan from Rhodotorula glutinis as an Immunoreactive Antigen in Diagnosis of Leptospirosis
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Utilization of Exocellular Mannan from Rhodotorula glutinis as an Immunoreactive Antigen in Diagnosis of Leptospirosis

机译：利用Rhodotorula glutinis胞外甘露聚糖作为免疫反应性抗原诊断钩端螺旋体病

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Previously, Rhodotorula glutinis was reported to produce a large amount of exocellular mannan, having a repeating unit of →3)-d-Manp-(1→4)-d-Manp-(1→. Recently, we found that antigenic polysaccharides ofLeptospira biflexa serovar patoc strain Patoc I have the same repeating unit and cross-react with antisera raised against extended strains of other leptospires (K. Matsuo, E. Isogai, and Y. Araki, Carbohydr. Res., in press). This structural identity and the difficulty of producing and isolating antigens led us to confirm the usefulness of Rhodotorula mannan as an immunoreactive antigen in a serological diagnosis of leptospirosis. In the present investigation, we confirmed the structural identity of an exocellular mannan isolated from R. glutinis AHU 3479 and tried to use it as an immunoreactive antigen in a serological diagnosis of leptospirosis. From its chemical analysis and 1H- and13C-labeled nuclear magnetic resonance spectrometry, theRhodotorula mannan was confirmed to consist of the same disaccharide units. Furthermore, such a preparation was shown to immunoreact to various sera from patients suffering with leptospirosis as well as to most rabbit antiserum preparations obtained from immunization with various strains of pathogenic leptospires. Therefore, the Rhodotorula mannan preparation is useful as an immunoreactive antigen in the serological diagnosis for leptospirosis.
机译：以前， Rhodotorula glutinis 据报道会产生大量的胞外甘露聚糖，其重复单元为→3）-d-Man p -（1→4）-d -Man p -（（1→。）。最近，我们发现 Leptospira biflexa 血清型Patoc株Patoc I的抗原多糖具有相同的重复单元，并与针对其的抗血清发生交叉反应。其他钩端螺旋体的延伸菌株（K. Matsuo，E. Isogai，和Y. Araki，Carbohydr。Res。，印刷中）。这种结构特征以及产生和分离抗原的困难使我们证实了 Rhodotorula 甘露聚糖作为钩端螺旋体病血清学诊断的一种免疫反应性抗原。化学检测和 1 H-和 13 C标记的核磁的免疫反应性抗原在钩端螺旋体病血清学诊断中的应用共振光谱法证实， Rhodotorula 甘露聚糖由相同的二糖单元组成。此外，显示这种制剂对患有钩端螺旋体病的患者的各种血清以及通过用各种病原性钩端螺旋体免疫接种获得的大多数兔抗血清制剂具有免疫反应。因此， Rhodotorula 甘露聚糖制剂可作为免疫反应性抗原用于钩端螺旋体病的血清学诊断。

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