Development of a Duplex PCR Genotyping Assay for Distinguishing Clostridium perfringens Type A Isolates Carrying Chromosomal Enterotoxin (cpe) Genes from Those Carrying Plasmid-Borne Enterotoxin (cpe) Genes

Qiyi Wen; Kazuaki Miyamoto; Bruce A. McClane

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Development of a Duplex PCR Genotyping Assay for Distinguishing Clostridium perfringens Type A Isolates Carrying Chromosomal Enterotoxin (cpe) Genes from Those Carrying Plasmid-Borne Enterotoxin (cpe) Genes

机译：用于区分产气荚膜梭状芽孢杆菌A型分离株携带染色体肠毒素（cpe）基因的双重PCR基因分型方法的开发

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About 5% of Clostridium perfringens type A isolates carry the cpe gene encoding the C. perfringens enterotoxin. Those cpe-positive type A isolates are important causes of food-poisoning and non-food-borne cases of diarrheas in humans, as well as certain veterinary cases of diarrhea. Previous studies have determined that the enterotoxigenic type A isolates causing both non-food-borne human gastrointestinal disease and veterinary disease carry their cpe genes on plasmids, while the type A isolates causing human food poisoning carry a chromosomal cpe gene. The present study reports on the successful development of a duplex PCR assay that can rapidly genotype enterotoxigenic type A isolates (i.e., determine whether those cpe-positive isolates carry a chromosomal or a plasmid-borne cpe gene). The availability of this rapid cpe genotyping assay capable of handling large numbers of samples provides a powerful new investigative tool for diagnostic, epidemiologic, and basic research purposes.

机译：大约5％的产气荚膜梭状芽孢杆菌分离株携带编码 C的 cpe 基因。产气荚膜毒素。那些 cpe 阳性的A型分离株是导致人中毒和非食物性腹泻以及某些兽医性腹泻的重要原因。先前的研究已经确定，引起非食源性人类胃肠道疾病和兽医疾病的产肠毒素A型分离株在质粒上带有 cpe 基因，而引起人类食物中毒的A型分离株具有染色体< em> cpe 基因。本研究报告了双链PCR测定法的成功开发，该测定法可快速鉴定基因型产毒型A型分离株（即确定那些 cpe 阳性分离株是否携带染色体或质粒携带的 cpe 基因）。能够处理大量样品的快速 cpe 基因分型分析的可用性为诊断，流行病学和基础研究目的提供了强大的新调查工具。 展开▼

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《Journal of Clinical Microbiology》 |2003年第4期|共5页

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Qiyi Wen; Kazuaki Miyamoto; Bruce A. McClane;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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1. Multiplex PCR Genotyping Assay That Distinguishes between Isolates of Clostridium perfringens Type A Carrying a Chromosomal Enterotoxin Gene (cpe) Locus, a Plasmid cpe Locus with an IS1470-Like Sequence, or a Plasmid cpe Locus with an IS1151 Sequence [J] . Kazuaki Miyamoto, Qiyi Wen, Bruce A. McClane Journal of Clinical Microbiology . 2004,第4期

机译：多重PCR基因分型分析，可区分携带细菌肠毒素基因（cpe）基因座，具有IS1470序列的质粒cpe基因座或具有IS1151序列的质粒cpe基因座的产气荚膜梭状芽孢杆菌分离株

2. Clostridium perfringens Type A Strains Carrying a Plasmid-Borne Enterotoxin Gene (Genotype IS1151-cpe or IS1470-like-cpe) as a Common Cause of Food Poisoning [J] . P?ivi Lahti, Annamari Heikinheimo, Tuula Johansson, Journal of Clinical Microbiology . 2008,第1期

机译：携带质粒-肠毒素基因（基因型IS1151-cpe或IS1470-like-cpe）的产气荚膜梭菌菌株是食物中毒的常见原因

3. In vitro cytotoxicity induced by Clostridium perfringens isolate carrying a chromosomal cpe gene is exclusively dependent on sporulation and enterotoxin production [J] . Yasugi Mayo, Sugahara Yuki, Hoshi Hidenobu, Microbial Pathogenesis . 2015,第Null期

机译：携带染色体cpe基因的产气荚膜梭菌分离物诱导的体外细胞毒性完全取决于孢子形成和肠毒素产生

4. A retrospective study of the prevalence of cpa, cpby cpe, and cpb2 toxin genes among Clostridium perfringens field isolates obtained from pigs with gastrointestinal diseases using PCR genotyping [C] . Boh Chang Lin Annual Meeting of the American Association of Swine Veterinarians . 2004

机译：使用PCR基因分型与胃肠疾病获得的梭菌野生植物场分离株的CPA，CPB CPE和CPB2毒素基因患病率的回顾性研究

5. Development of a Duplex PCR Genotyping Assay for Distinguishing Clostridium perfringens Type A Isolates Carrying Chromosomal Enterotoxin (cpe) Genes from Those Carrying Plasmid-Borne Enterotoxin (cpe) Genes [O] . Qiyi Wen, Kazuaki Miyamoto, Bruce A. McClane 2003

机译：用于区分产气荚膜梭状芽孢杆菌A型分离株携带染色体肠毒素（cpe）基因的双重PCR基因分型方法的开发

6. Development of a Duplex PCR Genotyping Assay for Distinguishing Clostridium perfringens Type A Isolates Carrying Chromosomal Enterotoxin (cpe) Genes from Those Carrying Plasmid-Borne Enterotoxin (cpe) Genes [O] . Wen, Qiyi, Miyamoto, Kazuaki, McClane, Bruce A. 2003

机译：用于区分产气荚膜梭状芽孢杆菌A型分离株携带染色体肠毒素（cpe）基因的双重PCR基因分型方法的开发

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2. A型产气荚膜梭菌青海分离株tetA(P)耐药基因序列分析及蛋白结构预测 [J] . 冶贵生 ,马玉花 ,张爽 . 动物医学进展 . 2015,第006期

3. A 型产气荚膜梭菌青海分离株α毒素基因分析及其蛋白结构与抗原表位预测 [J] . 冶贵生 ,马玉花 ,韩志辉 . 动物医学进展 . 2014,第008期

4. 四川地区鸡源产气荚膜梭菌的分离鉴定与基因分型 [J] . 倪学勤 ,宋振银 ,曾东 . 中国人兽共患病学报 . 2009,第008期

5. 致人腹泻产气荚膜梭菌的分离鉴定与基因分型 [J] . 张小荣 ,文其乙 ,刘秀梵 . 中国人兽共患病学报 . 2002,第004期

6. C型产气荚膜梭菌贵州株肠毒素基因克隆与序列分析 [C] . 唐源 ,文明 ,罗阿东 . 第二届全国微生物实用技术生态环境应用学术研讨会 . 2009

7. 应用多重PCR方法对产气荚膜梭菌及CPE阳性产气荚膜梭菌分离株的基因分型与鉴定 [A] . 赵李祥 . 2005

1. 葡萄球菌肠毒素基因型的检测方法及基因分型的检测方法 [P] . 中国专利： CN101760553A . 2010-06-30

2. 用于确定UGT1A基因的基因型的htSNP及其用于多重基因分型的方法 [P] . 中国专利： CN102277437B . 2015.03.25

3. cpa cpe Singleplex Real-Time PCR DEVELOPMENT OF SINGLEPLEX REAL-TIME PCR KIT FOR RAPID DETECTION OF CLOSTRIDIUM PERFRINGENS USING cpa cpe TARGET GENE [P] . 外国专利： KR20190065687A . 2019-06-12

机译：cpa cpe单重实时荧光定量PCR试剂盒的开发单核实时荧光定量PCR试剂盒，用于使用cpa cpe目标基因快速检测环孢菌

4. cpa cpe Singleplex Real-Time PCR DEVELOPMENT OF SINGLEPLEX REAL-TIME PCR KIT FOR RAPID DETECTION OF CLOSTRIDIUM PERFRINGENS USING cpa cpe TARGET GENE [P] . 外国专利： KR102009326B1 . 2019-08-12

机译：cpa cpe单重实时荧光定量PCR试剂盒的开发单核实时荧光定量PCR试剂盒，用于使用cpa cpe目标基因快速检测环孢菌

5. PRIMERS FOR THE AMPLIFICATION OF GENES CODING FOR THE ENTEROTOXIN AND THE LECITHINASE OF CLOSTRIDIUM PERFRINGENS AND THEIR APPLICATION TO THE DETECTION AND COUNTING OF THESE BACTERIAE [P] . 外国专利： EP0739420B1 . 2002-05-02

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Development of a Duplex PCR Genotyping Assay for Distinguishing Clostridium perfringens Type A Isolates Carrying Chromosomal Enterotoxin (cpe) Genes from Those Carrying Plasmid-Borne Enterotoxin (cpe) Genes

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